Gene description for H3f3b
Gene name H3 histone, family 3B
Gene symbol H3f3b
Other names/aliases 9430068D06Rik
H3.3B
Species Mus musculus
 Database cross references - H3f3b
ExoCarta ExoCarta_15081
Vesiclepedia VP_15081
Entrez Gene 15081
UniProt P84244  
 H3f3b identified in sEVs derived from the following tissue/cell type
Dendritic cells 11390481    
Fibroblasts 23260141    
Mast cells 17486113    
 Gene ontology annotations for H3f3b
Molecular Function
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 ISO
    RNA polymerase II core promoter sequence-specific DNA binding GO:0000979 ISO
    nucleosomal DNA binding GO:0031492 ISO
Biological Process
    osteoblast differentiation GO:0001649 IGI
    nucleus organization GO:0006997 IMP
    spermatogenesis GO:0007283 IMP
    spermatid development GO:0007286 IMP
    single fertilization GO:0007338 IMP
    embryo implantation GO:0007566 IMP
    cell population proliferation GO:0008283 IGI
    male gonad development GO:0008584 IMP
    pericentric heterochromatin formation GO:0031508 IGI
    subtelomeric heterochromatin formation GO:0031509 IGI
    multicellular organism growth GO:0035264 IMP
    muscle cell differentiation GO:0042692 IGI
    oogenesis GO:0048477 IGI
    regulation of centromere complex assembly GO:0090230 IMP
    negative regulation of chromosome condensation GO:1902340 IGI
Subcellular Localization
    chromosome, telomeric region GO:0000781 ISO
    nucleosome GO:0000786 ISO
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 ISO
    protein-containing complex GO:0032991 ISO
 Experiment description of studies that identified H3f3b in sEVs
1
Experiment ID 10
MISEV standards
EM
Biophysical techniques
Alix|CD9|MHCII|MHCI|CD86|LAMP2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [MALDI TOF]
PubMed ID 11390481    
Organism Mus musculus
Experiment description Proteomic analysis of dendritic cell-derived exosomes: a secreted subcellular compartment distinct from apoptotic vesicles.
Authors "Thery C, Boussac M, Veron P, Ricciardi-Castagnoli P, Raposo G, Garin J, Amigorena S."
Journal name JIMMU
Publication year 2001
Sample Dendritic cells
Sample name D1
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Mass spectrometry [QTOF]
Western blotting
2
Experiment ID 210
MISEV standards
EM
Biophysical techniques
CD81|FLOT1
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for H3f3b
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Hira  
Two-hybrid Mus musculus
Reconstituted Complex Mus musculus
2 Ccnb1ip1  
Two-hybrid Mus musculus
3 Unk  
Affinity Capture-RNA Mus musculus
View the network image/svg+xml



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