Gene description for Hars
Gene name histidyl-tRNA synthetase
Gene symbol Hars
Other names/aliases MMHRS
Species Mus musculus
 Database cross references - Hars
ExoCarta ExoCarta_15115
Entrez Gene 15115
UniProt Q61035  
 Hars identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Hars
Molecular Function
    nucleotide binding GO:0000166 IEA
    aminoacyl-tRNA ligase activity GO:0004812 IEA
    molecular_function GO:0003674 ND
    histidine-tRNA ligase activity GO:0004821 IBA
    ATP binding GO:0005524 IEA
    ligase activity GO:0016874 IEA
Biological Process
    mitochondrial translation GO:0032543 IBA
    histidyl-tRNA aminoacylation GO:0006427 IBA
    biological_process GO:0008150 ND
    tRNA aminoacylation for protein translation GO:0006418 IEA
    translation GO:0006412 IEA
Subcellular Localization
    mitochondrion GO:0005739 IBA
    cytoplasm GO:0005737 ISO
 Experiment description of studies that identified Hars in exosomes
1
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Hars
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Hars is involved
No pathways found





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