Gene description for Icam1
Gene name intercellular adhesion molecule 1
Gene symbol Icam1
Other names/aliases CD54
Icam-1
Ly-47
MALA-2
Species Mus musculus
 Database cross references - Icam1
ExoCarta ExoCarta_15894
Entrez Gene 15894
UniProt P13597  
 Icam1 identified in exosomes derived from the following tissue/cell type
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Mast cells 11145662    
Mast cells 11306949    
Mast cells 17486113    
 Gene ontology annotations for Icam1
Molecular Function
    integrin binding GO:0005178 ISO
    protein complex binding GO:0032403 ISO
Biological Process
    positive regulation of vasoconstriction GO:0045907 ISO
    receptor-mediated virion attachment to host cell GO:0046813 ISO
    positive regulation of nitric oxide biosynthetic process GO:0045429 ISO
    regulation of ruffle assembly GO:1900027 IDA
    positive regulation of cellular extravasation GO:0002693 ISO
    regulation of cell adhesion GO:0030155 IDA
    sensory perception of sound GO:0007605 ISO
    adhesion of symbiont to host GO:0044406 ISO
    negative regulation of extrinsic apoptotic signaling pathway via death domain receptors GO:1902042 ISO
    positive regulation of NF-kappaB transcription factor activity GO:0051092 ISO
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 ISO
    cell adhesion mediated by integrin GO:0033627 IDA
    single organismal cell-cell adhesion GO:0016337 ISO
    regulation of cell shape GO:0008360 ISO
    positive regulation of GTPase activity GO:0043547 ISO
    negative regulation of endothelial cell apoptotic process GO:2000352 ISO
    establishment of endothelial barrier GO:0061028 ISO
    positive regulation of actin filament polymerization GO:0030838 ISO
    cell adhesion GO:0007155 ISO
    negative regulation of calcium ion transport GO:0051926 ISO
    cellular response to glucose stimulus GO:0071333 IDA
    leukocyte cell-cell adhesion GO:0007159 ISO
    positive regulation of ERK1 and ERK2 cascade GO:0070374 ISO
    establishment of Sertoli cell barrier GO:0097368 ISO
    acute inflammatory response to antigenic stimulus GO:0002438 ISO
    T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell GO:0002291 ISO
    T cell antigen processing and presentation GO:0002457 IDA
Subcellular Localization
    extracellular space GO:0005615 ISO
    membrane GO:0016020 ISO
    extracellular exosome GO:0070062 ISO
    integral component of membrane GO:0016021 IEA
    cell surface GO:0009986 ISO
    membrane raft GO:0045121 IDA
    integral component of plasma membrane GO:0005887 IBA
    focal adhesion GO:0005925 ISO
    plasma membrane GO:0005886 ISO
    immunological synapse GO:0001772 IDA
    external side of plasma membrane GO:0009897 IDA
 Experiment description of studies that identified Icam1 in exosomes
1
Experiment ID 214
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 215
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 216
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 5
ISEV standards
EM|IEM
EV Biophysical techniques
EV Cytosolic markers
CD86|CD80
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method ELISA
PubMed ID 11145662    
Organism Mus musculus
Experiment description Mast cell-dependent B and T lymphocyte activation is mediated by the secretion of immunologically active exosomes.
Authors Skokos D, Le Panse S, Villa I, Rousselle JC, Peronet R, David B, Namane A, Mecheri S
Journal name JIMMU
Publication year 2001
Sample Mast cells
Sample name P815
MC9
Bone marrow-derived mast cells
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
ELISA
5
Experiment ID 6
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 11306949    
Organism Mus musculus
Experiment description Nonspecific B and T cell-stimulatory activity mediated by mast cells is associated with exosomes.
Authors Skokos D, Le Panse S, Villa I, Rousselle JC, Peronet R, Namane A, David B, Mecheri S
Journal name IAAI
Publication year 2001
Sample Mast cells
Sample name Bone marrow-derived mast cells
Isolation/purification methods -
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
6
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Icam1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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