Gene description for Jchain
Gene name immunoglobulin joining chain
Gene symbol Jchain
Other names/aliases 9530090F24Rik
AI323815
Igj
Jch
Species Mus musculus
 Database cross references - Jchain
ExoCarta ExoCarta_16069
Vesiclepedia VP_16069
Entrez Gene 16069
UniProt P01592  
 Jchain identified in sEVs derived from the following tissue/cell type
Faeces 33431899    
Faeces 33431899    
 Gene ontology annotations for Jchain
Molecular Function
    single-stranded DNA binding GO:0003697 IEA
    single-stranded DNA binding GO:0003697 ISO
    antigen binding GO:0003823 ISS
    IgA binding GO:0019862 IDA
    IgA binding GO:0019862 ISO
    protein-macromolecule adaptor activity GO:0030674 IDA
    phosphatidylcholine binding GO:0031210 IEA
    phosphatidylcholine binding GO:0031210 ISO
    immunoglobulin receptor binding GO:0034987 IBA
    immunoglobulin receptor binding GO:0034987 IMP
    immunoglobulin receptor binding GO:0034987 ISO
    protein homodimerization activity GO:0042803 IEA
    protein homodimerization activity GO:0042803 ISO
    peptidoglycan binding GO:0042834 IEA
    peptidoglycan binding GO:0042834 ISO
Biological Process
    adaptive immune response GO:0002250 IEA
    adaptive immune response GO:0002250 ISO
    glomerular filtration GO:0003094 IEA
    glomerular filtration GO:0003094 ISO
    humoral immune response GO:0006959 IBA
    humoral immune response GO:0006959 ISS
    antibacterial humoral response GO:0019731 IEA
    antibacterial humoral response GO:0019731 ISO
    innate immune response GO:0045087 IEA
    innate immune response GO:0045087 ISO
    positive regulation of respiratory burst GO:0060267 IEA
    positive regulation of respiratory burst GO:0060267 ISO
    protein-containing complex assembly GO:0065003 IEA
    protein-containing complex assembly GO:0065003 ISO
Subcellular Localization
    extracellular space GO:0005615 ISO
    monomeric IgA immunoglobulin complex GO:0071748 IEA
    monomeric IgA immunoglobulin complex GO:0071748 ISO
    dimeric IgA immunoglobulin complex GO:0071750 IMP
    dimeric IgA immunoglobulin complex GO:0071750 ISO
    secretory IgA immunoglobulin complex GO:0071751 ISO
    secretory IgA immunoglobulin complex GO:0071751 ISS
    secretory dimeric IgA immunoglobulin complex GO:0071752 IEA
    secretory dimeric IgA immunoglobulin complex GO:0071752 ISO
    pentameric IgM immunoglobulin complex GO:0071756 IBA
    pentameric IgM immunoglobulin complex GO:0071756 ISO
    pentameric IgM immunoglobulin complex GO:0071756 ISS
 Experiment description of studies that identified Jchain in sEVs
1
Experiment ID 303
MISEV standards
EM
Biophysical techniques
Cd63|Cd81|Cd9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
2
Experiment ID 304
MISEV standards
EM
Biophysical techniques
Cd63|Cd81|Cd9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
 Protein-protein interactions for Jchain
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fancd2  
Affinity Capture-MS Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here