Gene description for Itga4
Gene name integrin alpha 4
Gene symbol Itga4
Other names/aliases CD49D
Itga4B
Species Mus musculus
 Database cross references - Itga4
ExoCarta ExoCarta_16401
Entrez Gene 16401
UniProt Q00651  
 Itga4 identified in exosomes derived from the following tissue/cell type
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
 Gene ontology annotations for Itga4
Molecular Function
    protein antigen binding GO:1990405 ISO
    metal ion binding GO:0046872 IEA
    cell adhesion molecule binding GO:0050839 ISO
    fibronectin binding GO:0001968 ISO
Biological Process
    diapedesis GO:0050904 ISO
    visceral serous pericardium development GO:0061032 TAS
    leukocyte tethering or rolling GO:0050901 ISO
    mesenchyme development GO:0060485 TAS
    positive regulation of T cell migration GO:2000406 ISO
    leukocyte cell-cell adhesion GO:0007159 ISO
    cell-matrix adhesion GO:0007160 TAS
    cell migration GO:0016477 IMP
    heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules GO:0007157 IMP
    positive regulation of leukocyte migration GO:0002687 ISO
    heterotypic cell-cell adhesion GO:0034113 ISO
    face development GO:0060324 IMP
    T cell migration GO:0072678 IMP
    positive regulation of leukocyte tethering or rolling GO:1903238 ISO
    receptor clustering GO:0043113 ISO
    import into cell GO:0098657 ISO
    negative regulation of protein homodimerization activity GO:0090074 ISO
    chorio-allantoic fusion GO:0060710 IMP
    blood vessel remodeling GO:0001974 IMP
    positive regulation of leukocyte cell-cell adhesion GO:1903039 ISO
    cell adhesion GO:0007155 IMP
    substrate adhesion-dependent cell spreading GO:0034446 ISO
    cell-matrix adhesion involved in ameboidal cell migration GO:0003366 ISO
    heart development GO:0007507 IMP
    integrin-mediated signaling pathway GO:0007229 IEA
    single organismal cell-cell adhesion GO:0016337 TAS
Subcellular Localization
    integrin complex GO:0008305 IEA
    cell surface GO:0009986 TAS
    membrane GO:0016020 ISO
    integrin alpha4-beta7 complex GO:0034669 ISO
    integral component of membrane GO:0016021 IEA
    external side of plasma membrane GO:0009897 IDA
    plasma membrane GO:0005886 ISO
    cell-cell junction GO:0005911 IDA
    extracellular exosome GO:0070062 ISO
    focal adhesion GO:0005925 ISO
 Experiment description of studies that identified Itga4 in exosomes
1
Experiment ID 214
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 215
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 216
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Itga4
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Itga4 is involved
No pathways found





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