Gene description for Itgam
Gene name integrin alpha M
Gene symbol Itgam
Other names/aliases CD11b/CD18
CR3
CR3A
Cd11b
F730045J24Rik
Ly-40
MAC1
Mac-1
Mac-1a
Species Mus musculus
 Database cross references - Itgam
ExoCarta ExoCarta_16409
Entrez Gene 16409
 Itgam identified in exosomes derived from the following tissue/cell type
Dendritic cells 10545503    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Microglia 16081791    
 Gene ontology annotations for Itgam
Molecular Function
    heparin binding GO:0008201 IDA
    heparan sulfate proteoglycan binding GO:0043395 IDA
    protein heterodimerization activity GO:0046982 ISO
    opsonin binding GO:0001846 IMP
    metal ion binding GO:0046872 IEA
    glycoprotein binding GO:0001948 ISO
Biological Process
    single organismal cell-cell adhesion GO:0016337 IGI
    microglia development GO:0014005 IDA
    cellular extravasation GO:0045123 IMP
    leukocyte cell-cell adhesion GO:0007159 IMP
    neutrophil chemotaxis GO:0030593 IMP
    cell adhesion GO:0007155 IMP
    integrin-mediated signaling pathway GO:0007229 IEA
    activated T cell proliferation GO:0050798 IMP
    leukocyte migration involved in inflammatory response GO:0002523 IMP
Subcellular Localization
    cell surface GO:0009986 ISO
    extracellular exosome GO:0070062 ISO
    nucleus GO:0005634 IDA
    external side of plasma membrane GO:0009897 IDA
    integral component of membrane GO:0016021 IDA
    integrin complex GO:0008305 IEA
    membrane GO:0016020 IEA
 Experiment description of studies that identified Itgam in exosomes
1
Experiment ID 3
ISEV standards
IEM
EV Biophysical techniques
HSP84|HSC73
EV Cytosolic markers
MHCII|CD9|mac-1
EV Membrane markers
CANX|HSP90B1
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
Western blotting
PubMed ID 10545503    
Organism Mus musculus
Experiment description Molecular characterization of dendritic cell-derived exosomes. Selective accumulation of the heat shock protein hsc73.
Authors Thery C, Regnault A, Garin J, Wolfers J, Zitvogel L, Ricciardi-Castagnoli P, Raposo G, Amigorena S.
Journal name JCB
Publication year 1999
Sample Dendritic cells
Sample name D1
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
2
Experiment ID 214
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 215
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 216
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 14
ISEV standards
EM
EV Biophysical techniques
RAB7|RAB11
EV Cytosolic markers
CD9|CD63|LAMP1|LAMP2
EV Membrane markers
DNM
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 16081791    
Organism Mus musculus
Experiment description Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism.
Authors Potolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ, Santambrogio L
Journal name JIMMU
Publication year 2005
Sample Microglia
Sample name N9
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
 Protein-protein interactions for Itgam
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Itgam is involved
No pathways found





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