Gene description for DDC
Gene name dopa decarboxylase (aromatic L-amino acid decarboxylase)
Gene symbol DDC
Other names/aliases AADC
Species Homo sapiens
 Database cross references - DDC
ExoCarta ExoCarta_1644
Vesiclepedia VP_1644
Entrez Gene 1644
HGNC 2719
MIM 107930
UniProt P20711  
 DDC identified in sEVs derived from the following tissue/cell type
Urine 19056867    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for DDC
Molecular Function
    aromatic-L-amino-acid decarboxylase activity GO:0004058 IBA
    aromatic-L-amino-acid decarboxylase activity GO:0004058 IDA
    protein binding GO:0005515 IPI
    enzyme binding GO:0019899 IPI
    pyridoxal phosphate binding GO:0030170 IEA
    5-hydroxy-L-tryptophan decarboxylase activity GO:0036467 IDA
    L-dopa decarboxylase activity GO:0036468 IDA
Biological Process
    kidney development GO:0001822 IEA
    amino acid metabolic process GO:0006520 IEA
    catecholamine metabolic process GO:0006584 IBA
    response to toxic substance GO:0009636 IEA
    gene expression GO:0010467 IEA
    carboxylic acid metabolic process GO:0019752 IEA
    dopamine biosynthetic process GO:0042416 IDA
    dopamine biosynthetic process GO:0042416 IEA
    serotonin biosynthetic process GO:0042427 IBA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified DDC in sEVs
1
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
2
Experiment ID 196
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 197
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for DDC
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ATF6  
Two-hybrid Homo sapiens
2 BSG 682
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 RELA 5970
Two-hybrid Homo sapiens
View the network image/svg+xml



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