Gene description for Khsrp
Gene name KH-type splicing regulatory protein
Gene symbol Khsrp
Other names/aliases 6330409F21Rik
Fbp2
Fubp2
Ksrp
Species Mus musculus
 Database cross references - Khsrp
ExoCarta ExoCarta_16549
Vesiclepedia VP_16549
Entrez Gene 16549
UniProt Q3U0V1  
 Khsrp identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Khsrp
Molecular Function
    DNA binding GO:0003677 IEA
    mRNA binding GO:0003729 IBA
    mRNA binding GO:0003729 IDA
    mRNA 3'-UTR binding GO:0003730 ISO
    mRNA 3'-UTR AU-rich region binding GO:0035925 ISO
    mRNA 3'-UTR AU-rich region binding GO:0035925 ISS
    protein folding chaperone GO:0044183 ISO
Biological Process
    regulation of DNA-templated transcription GO:0006355 IEA
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    mRNA processing GO:0006397 IEA
    mRNA catabolic process GO:0006402 IDA
    RNA splicing GO:0008380 IEA
    miRNA metabolic process GO:0010586 ISO
    miRNA metabolic process GO:0010586 ISS
    negative regulation of low-density lipoprotein particle clearance GO:0010989 ISO
    regulation of mRNA stability GO:0043488 ISO
    regulation of mRNA stability GO:0043488 ISS
    negative regulation of nitric oxide biosynthetic process GO:0045019 ISO
    negative regulation of nitric oxide biosynthetic process GO:0045019 ISS
    mRNA transport GO:0051028 IEA
    positive regulation of mRNA catabolic process GO:0061014 ISO
    positive regulation of mRNA catabolic process GO:0061014 ISS
    3'-UTR-mediated mRNA destabilization GO:0061158 ISO
    3'-UTR-mediated mRNA destabilization GO:0061158 ISS
    cellular response to cytokine stimulus GO:0071345 ISO
    cellular response to cytokine stimulus GO:0071345 ISS
    regulation of miRNA metabolic process GO:2000628 IDA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
    cytoplasmic stress granule GO:0010494 IDA
    dendrite GO:0030425 ISO
    neuronal cell body GO:0043025 ISO
 Experiment description of studies that identified Khsrp in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Khsrp
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Invs  
Affinity Capture-MS Mus musculus
2 Tial1  
Co-fractionation Mus musculus
3 Fmr1  
Affinity Capture-MS Mus musculus
Co-fractionation Mus musculus
4 G3bp1  
Co-fractionation Mus musculus
5 Kctd13  
Affinity Capture-MS Mus musculus
6 Traf3  
Proximity Label-MS Mus musculus
7 Atxn1  
Proximity Label-MS Mus musculus
Affinity Capture-MS Mus musculus
8 FBXW2  
Affinity Capture-MS Homo sapiens
9 Tia1  
Co-fractionation Mus musculus
10 Stau1  
Co-fractionation Mus musculus
11 Fancd2  
Affinity Capture-MS Mus musculus
12 Ptbp2  
Co-fractionation Mus musculus
13 Rae1  
Co-fractionation Mus musculus
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