Gene description for Kras
Gene name v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Gene symbol Kras
Other names/aliases AI929937
K-ras
Ki-ras
Kras-2
Kras2
p21B
ras
Species Mus musculus
 Database cross references - Kras
ExoCarta ExoCarta_16653
Vesiclepedia VP_16653
Entrez Gene 16653
UniProt P32883  
 Kras identified in sEVs derived from the following tissue/cell type
Embryonic fibroblasts 18494037    
Mast cells 17486113    
 Gene ontology annotations for Kras
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 IGI
    GTPase activity GO:0003924 ISO
    GTPase activity GO:0003924 TAS
    G protein activity GO:0003925 IEA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IBA
    GTP binding GO:0005525 IEA
    GMP binding GO:0019002 ISO
    GDP binding GO:0019003 IBA
    GDP binding GO:0019003 ISO
    LRR domain binding GO:0030275 ISO
    identical protein binding GO:0042802 ISO
    protein-membrane adaptor activity GO:0043495 IDA
    protein-containing complex binding GO:0044877 ISO
Biological Process
    positive regulation of protein phosphorylation GO:0001934 ISO
    Ras protein signal transduction GO:0007265 IBA
    Ras protein signal transduction GO:0007265 IGI
    Ras protein signal transduction GO:0007265 IMP
    Ras protein signal transduction GO:0007265 TAS
    cell population proliferation GO:0008283 IGI
    visual learning GO:0008542 IGI
    gene expression GO:0010467 IMP
    positive regulation of gene expression GO:0010628 IMP
    positive regulation of gene expression GO:0010628 ISO
    glial cell proliferation GO:0014009 IGI
    Rac protein signal transduction GO:0016601 IGI
    cytokine-mediated signaling pathway GO:0019221 ISO
    forebrain astrocyte development GO:0021897 IMP
    actin cytoskeleton organization GO:0030036 IGI
    negative regulation of epithelial cell differentiation GO:0030857 IDA
    regulation of synaptic transmission, GABAergic GO:0032228 IGI
    positive regulation of Rac protein signal transduction GO:0035022 IGI
    skeletal muscle cell differentiation GO:0035914 IGI
    negative regulation of neuron apoptotic process GO:0043524 IGI
    insulin-like growth factor receptor signaling pathway GO:0048009 ISO
    regulation of long-term neuronal synaptic plasticity GO:0048169 IGI
    homeostasis of number of cells within a tissue GO:0048873 IGI
    striated muscle cell differentiation GO:0051146 IGI
    neuron apoptotic process GO:0051402 IGI
    positive regulation of glial cell proliferation GO:0060252 IGI
    epithelial tube branching involved in lung morphogenesis GO:0060441 IDA
    epithelial tube branching involved in lung morphogenesis GO:0060441 IGI
    type I pneumocyte differentiation GO:0060509 IDA
    positive regulation of cellular senescence GO:2000774 ISO
Subcellular Localization
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 TAS
    cytoplasmic side of plasma membrane GO:0009898 ISO
    cytoplasmic side of plasma membrane GO:0009898 ISS
    endomembrane system GO:0012505 IEA
    membrane GO:0016020 IDA
    membrane GO:0016020 IGI
 Experiment description of studies that identified Kras in sEVs
1
Experiment ID 73
MISEV standards
EM
Biophysical techniques
HSP70|HSP90
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors "Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ"
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
2
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Kras
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fancd2  
Affinity Capture-MS Mus musculus
2 Atg16l1  
Affinity Capture-MS Mus musculus
3 Tsc1  
Affinity Capture-MS Mus musculus
4 Traf3  
Proximity Label-MS Mus musculus
5 Unk  
Affinity Capture-RNA Mus musculus
View the network image/svg+xml
 Pathways in which Kras is involved
PathwayEvidenceSource
Activation of RAS in B cells IEA Reactome
Adaptive Immune System IEA Reactome
Axon guidance IEA Reactome
Beta-catenin independent WNT signaling IEA Reactome
C-type lectin receptors (CLRs) IEA Reactome
Ca2+ pathway IEA Reactome
CD209 (DC-SIGN) signaling IEA Reactome
Cell surface interactions at the vascular wall IEA Reactome
Cytokine Signaling in Immune system IEA Reactome
DAP12 interactions IEA Reactome
DAP12 signaling IEA Reactome
Developmental Biology IEA Reactome
Downstream signal transduction IEA Reactome
Downstream signaling events of B Cell Receptor (BCR) IEA Reactome
Downstream signaling of activated FGFR1 IEA Reactome
Downstream signaling of activated FGFR2 IEA Reactome
Downstream signaling of activated FGFR3 IEA Reactome
Downstream signaling of activated FGFR4 IEA Reactome
EGFR Transactivation by Gastrin IEA Reactome
ESR-mediated signaling IEA Reactome
Estrogen-stimulated signaling through PRKCZ IEA Reactome
Extra-nuclear estrogen signaling IEA Reactome
Fc epsilon receptor (FCERI) signaling IEA Reactome
FCERI mediated MAPK activation IEA Reactome
FLT3 Signaling IEA Reactome
FRS-mediated FGFR1 signaling IEA Reactome
FRS-mediated FGFR2 signaling IEA Reactome
FRS-mediated FGFR3 signaling IEA Reactome
FRS-mediated FGFR4 signaling IEA Reactome
G alpha (q) signalling events IEA Reactome
Gastrin-CREB signalling pathway via PKC and MAPK IEA Reactome
GPCR downstream signalling IEA Reactome
GRB2 events in EGFR signaling IEA Reactome
GRB2 events in ERBB2 signaling IEA Reactome
Hemostasis IEA Reactome
Immune System IEA Reactome
Innate Immune System IEA Reactome
MAP2K and MAPK activation IEA Reactome
MAPK family signaling cascades IEA Reactome
MAPK1/MAPK3 signaling IEA Reactome
MET activates RAS signaling IEA Reactome
NCAM signaling for neurite out-growth IEA Reactome
Negative regulation of MAPK pathway IEA Reactome
Nervous system development IEA Reactome
p38MAPK events IEA Reactome
PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases IEA Reactome
RAF activation IEA Reactome
RAF/MAP kinase cascade IEA Reactome
RAS processing IEA Reactome
Regulation of RAS by GAPs IEA Reactome
SHC-mediated cascade:FGFR1 IEA Reactome
SHC-mediated cascade:FGFR2 IEA Reactome
SHC-mediated cascade:FGFR3 IEA Reactome
SHC-mediated cascade:FGFR4 IEA Reactome
SHC1 events in EGFR signaling IEA Reactome
SHC1 events in ERBB4 signaling IEA Reactome
Signal Transduction IEA Reactome
Signaling by CSF3 (G-CSF) IEA Reactome
Signaling by EGFR IEA Reactome
Signaling by ERBB2 IEA Reactome
Signaling by ERBB4 IEA Reactome
Signaling by FGFR IEA Reactome
Signaling by FGFR1 IEA Reactome
Signaling by FGFR2 IEA Reactome
Signaling by FGFR3 IEA Reactome
Signaling by FGFR4 IEA Reactome
Signaling by GPCR IEA Reactome
Signaling by MET IEA Reactome
Signaling by Non-Receptor Tyrosine Kinases IEA Reactome
Signaling by NTRK1 (TRKA) IEA Reactome
Signaling by NTRKs IEA Reactome
Signaling by Nuclear Receptors IEA Reactome
Signaling by PDGF IEA Reactome
Signaling by PTK6 IEA Reactome
Signaling by Receptor Tyrosine Kinases IEA Reactome
Signaling by SCF-KIT IEA Reactome
Signaling by the B Cell Receptor (BCR) IEA Reactome
Signaling by VEGF IEA Reactome
Signaling by WNT IEA Reactome
Signalling to ERKs IEA Reactome
Signalling to RAS IEA Reactome
Tie2 Signaling IEA Reactome
VEGFA-VEGFR2 Pathway IEA Reactome
VEGFR2 mediated cell proliferation IEA Reactome





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