Gene description for Krt10
Gene name keratin 10
Gene symbol Krt10
Other names/aliases D130054E02Rik
K10
K1C1
Krt-1.10
Krt1-10
Species Mus musculus
 Database cross references - Krt10
ExoCarta ExoCarta_16661
Vesiclepedia VP_16661
Entrez Gene 16661
UniProt P02535  
 Krt10 identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
Oligodendrocytes 21136642    
 Gene ontology annotations for Krt10
Molecular Function
    structural molecule activity GO:0005198 IEA
    protein binding GO:0005515 IPI
    cytoskeletal protein binding GO:0008092 IGI
    structural constituent of skin epidermis GO:0030280 IBA
    structural constituent of skin epidermis GO:0030280 ISO
    protein heterodimerization activity GO:0046982 ISO
    protein heterodimerization activity GO:0046982 ISS
Biological Process
    keratinocyte development GO:0003334 IGI
    epidermis development GO:0008544 IBA
    epidermis development GO:0008544 IGI
    peptide cross-linking GO:0018149 ISO
    keratinocyte differentiation GO:0030216 IDA
    epithelial cell differentiation GO:0030855 IBA
    intermediate filament organization GO:0045109 IBA
    intermediate filament organization GO:0045109 IGI
    positive regulation of epidermis development GO:0045684 IMP
    stem cell differentiation GO:0048863 IDA
    protein heterotetramerization GO:0051290 ISO
    protein heterotetramerization GO:0051290 ISS
    cellular response to calcium ion GO:0071277 IDA
Subcellular Localization
    cornified envelope GO:0001533 IDA
    cornified envelope GO:0001533 ISO
    extracellular region GO:0005576 IEA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    cytoskeleton GO:0005856 IBA
    cell surface GO:0009986 IEA
    keratin filament GO:0045095 IBA
    keratin filament GO:0045095 IDA
    keratin filament GO:0045095 ISO
 Experiment description of studies that identified Krt10 in sEVs
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
2
Experiment ID 33
MISEV standards
EM|IEM
Biophysical techniques
Alix|TSG101
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors "Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J"
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for Krt10
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Nphp3  
Affinity Capture-MS Mus musculus
2 Invs  
Affinity Capture-MS Mus musculus
3 Kctd13  
Affinity Capture-MS Mus musculus
4 Cfap57  
Co-fractionation Mus musculus
5 Nphp4  
Affinity Capture-MS Mus musculus
6 Evpl  
Co-fractionation Mus musculus
7 Atxn1  
Proximity Label-MS Mus musculus
Affinity Capture-MS Mus musculus
8 Eed  
Affinity Capture-MS Mus musculus
9 Thrb  
Affinity Capture-MS Mus musculus
10 Ahi1  
Affinity Capture-MS Mus musculus
11 Nphp1  
Affinity Capture-MS Mus musculus
12 Mks1  
Affinity Capture-MS Mus musculus
13 Iqcb1  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Krt10 is involved
PathwayEvidenceSource
Developmental Biology IEA Reactome
Formation of the cornified envelope IEA Reactome
Keratinization IEA Reactome





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