Gene description for Lamp1
Gene name lysosomal-associated membrane protein 1
Gene symbol Lamp1
Other names/aliases AI196048
CD107a
Lamp-1
Species Mus musculus
 Database cross references - Lamp1
ExoCarta ExoCarta_16783
Vesiclepedia VP_16783
Entrez Gene 16783
UniProt P11438  
 Lamp1 identified in sEVs derived from the following tissue/cell type
Fibroblasts 23260141    
Mammary adenocarcinoma cells 11231627    
Melanoma cells 34957415    
Melanoma cells 34957415    
Melanoma cells 34957415    
Microglia 16081791    
 Gene ontology annotations for Lamp1
Molecular Function
    protein binding GO:0005515 IPI
    ion channel inhibitor activity GO:0008200 ISO
    ion channel inhibitor activity GO:0008200 ISS
    enzyme binding GO:0019899 IEA
    enzyme binding GO:0019899 ISO
    protein domain specific binding GO:0019904 IPI
Biological Process
    lysosomal lumen acidification GO:0007042 ISO
    lysosomal lumen acidification GO:0007042 ISS
    spermatogenesis GO:0007283 TAS
    positive regulation of natural killer cell degranulation GO:0043323 IEA
    positive regulation of natural killer cell degranulation GO:0043323 ISO
    positive regulation of natural killer cell mediated cytotoxicity GO:0045954 ISO
    autophagic cell death GO:0048102 ISO
    protein stabilization GO:0050821 IMP
    establishment of protein localization to organelle GO:0072594 IBA
    establishment of protein localization to organelle GO:0072594 IEA
    establishment of protein localization to organelle GO:0072594 ISO
    Golgi to lysosome transport GO:0090160 IEA
    Golgi to lysosome transport GO:0090160 ISO
    granzyme-mediated programmed cell death signaling pathway GO:0140507 IEA
    regulation of organelle transport along microtubule GO:1902513 IEA
    regulation of organelle transport along microtubule GO:1902513 ISO
Subcellular Localization
    autophagosome membrane GO:0000421 IDA
    cytoplasm GO:0005737 ISO
    lysosome GO:0005764 IDA
    lysosome GO:0005764 IDA
    lysosome GO:0005764 ISO
    lysosome GO:0005764 ISO
    lysosomal membrane GO:0005765 IBA
    lysosomal membrane GO:0005765 ISO
    endosome GO:0005768 IDA
    late endosome GO:0005770 IDA
    late endosome GO:0005770 ISO
    multivesicular body GO:0005771 IDA
    multivesicular body GO:0005771 ISO
    cytosol GO:0005829 IEA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISO
    synaptic vesicle GO:0008021 IDA
    external side of plasma membrane GO:0009897 IDA
    cell surface GO:0009986 IDA
    cell surface GO:0009986 IDA
    endosome membrane GO:0010008 ISS
    dendrite GO:0030425 ISO
    synaptic vesicle membrane GO:0030672 ISO
    late endosome membrane GO:0031902 IBA
    late endosome membrane GO:0031902 IEA
    vesicle GO:0031982 IDA
    sarcolemma GO:0042383 IDA
    melanosome GO:0042470 IDA
    neuronal cell body GO:0043025 ISO
    cytolytic granule GO:0044194 IDA
    autolysosome GO:0044754 IDA
    phagocytic vesicle GO:0045335 IMP
    perinuclear region of cytoplasm GO:0048471 IEA
    perinuclear region of cytoplasm GO:0048471 ISO
    phagolysosome membrane GO:0061474 IDA
    alveolar lamellar body GO:0097208 ISO
    cytolytic granule membrane GO:0101004 ISO
    cytolytic granule membrane GO:0101004 ISS
 Experiment description of studies that identified Lamp1 in sEVs
1
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 23
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
Immunoelectron Microscopy
PubMed ID 11231627    
Organism Mus musculus
Experiment description Tumor-derived exosomes are a source of shared tumor rejection antigens for CTL cross-priming.
Authors "Wolfers J, Lozier A, Raposo G, Regnault A, Thery C, Masurier C, Flament C, Pouzieux S, Faure F, Tursz T, Angevin E, Amigorena S, Zitvogel L"
Journal name NM
Publication year 2001
Sample Mammary adenocarcinoma cells
Sample name TS/A (H-2d)
P815 mastocytoma cells (H-2d)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Immunoelectron Microscopy
3
Experiment ID 1101
MISEV standards
✔
EM
Biophysical techniques
✔
Cd81|Cd9|Tfrc|Gapdh|Lamp1
Enriched markers
✔
GOLGA2|CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 1102
MISEV standards
✔
EM
Biophysical techniques
✔
Cd81|Cd9|Tfrc|Gapdh|Lamp1
Enriched markers
✔
GOLGA2|CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
Flow cytometry
5
Experiment ID 1103
MISEV standards
✔
EM
Biophysical techniques
✔
Cd81|Cd9|Tfrc|Gapdh|Lamp1
Enriched markers
✔
GOLGA2|CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
Flow cytometry
6
Experiment ID 14
MISEV standards
✔
EM
Biophysical techniques
✔
RAB7|RAB11|CD9|CD63|LAMP1|LAMP2
Enriched markers
✔
DNM
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 16081791    
Organism Mus musculus
Experiment description Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism.
Authors "Potolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ, Santambrogio L"
Journal name JIMMU
Publication year 2005
Sample Microglia
Sample name N9
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
 Protein-protein interactions for Lamp1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Lamp1 is involved
PathwayEvidenceSource
Immune System IEA Reactome
Innate Immune System IEA Reactome
Neutrophil degranulation IEA Reactome





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