Gene description for Anpep
Gene name alanyl (membrane) aminopeptidase
Gene symbol Anpep
Other names/aliases AP-M
AP-N
Apn
Cd13
P150
Species Mus musculus
 Database cross references - Anpep
ExoCarta ExoCarta_16790
Entrez Gene 16790
UniProt P97449  
 Anpep identified in exosomes derived from the following tissue/cell type
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Mast cells 11145662    
Mast cells 17486113    
Microglia 16081791    
 Gene ontology annotations for Anpep
Molecular Function
    hydrolase activity GO:0016787 IEA
    metallopeptidase activity GO:0008237 IEA
    aminopeptidase activity GO:0004177 ISO
    peptidase activity GO:0008233 IEA
    zinc ion binding GO:0008270 IEA
    metal ion binding GO:0046872 IEA
    peptide binding GO:0042277 ISO
Biological Process
    angiogenesis GO:0001525 IEA
    cell differentiation GO:0030154 IEA
    negative regulation of renal sodium excretion GO:0035814 ISO
    cellular aromatic compound metabolic process GO:0006725 ISO
    proteolysis GO:0006508 ISO
    multicellular organismal development GO:0007275 IEA
Subcellular Localization
    lysosomal membrane GO:0005765 ISO
    integral component of membrane GO:0016021 IEA
    vesicle membrane GO:0012506 ISO
    extracellular exosome GO:0070062 ISO
    extracellular space GO:0005615 ISO
    vesicle lumen GO:0031983 ISO
    endoplasmic reticulum-Golgi intermediate compartment GO:0005793 ISO
    external side of plasma membrane GO:0009897 IDA
    membrane GO:0016020 IEA
 Experiment description of studies that identified Anpep in exosomes
1
Experiment ID 214
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 215
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 216
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 5
ISEV standards
EM|IEM
EV Biophysical techniques
EV Cytosolic markers
CD86|CD80
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 11145662    
Organism Mus musculus
Experiment description Mast cell-dependent B and T lymphocyte activation is mediated by the secretion of immunologically active exosomes.
Authors Skokos D, Le Panse S, Villa I, Rousselle JC, Peronet R, David B, Namane A, Mecheri S
Journal name JIMMU
Publication year 2001
Sample Mast cells
Sample name P815
MC9
Bone marrow-derived mast cells
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
ELISA
5
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
6
Experiment ID 14
ISEV standards
EM
EV Biophysical techniques
RAB7|RAB11
EV Cytosolic markers
CD9|CD63|LAMP1|LAMP2
EV Membrane markers
DNM
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 16081791    
Organism Mus musculus
Experiment description Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism.
Authors Potolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ, Santambrogio L
Journal name JIMMU
Publication year 2005
Sample Microglia
Sample name N9
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
 Protein-protein interactions for Anpep
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Anpep is involved
PathwayEvidenceSource
Metabolism of Angiotensinogen to Angiotensins IEA Reactome





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