Gene description for Acaa2
Gene name acetyl-CoA acyltransferase 2
Gene symbol Acaa2
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Acaa2
ExoCarta ExoCarta_170465
Vesiclepedia VP_170465
Entrez Gene 170465
UniProt P13437  
 Acaa2 identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
Hepatocytes 19367702    
Reticulocytes 21828046    
 Gene ontology annotations for Acaa2
Molecular Function
    acetyl-CoA C-acetyltransferase activity GO:0003985 IBA
    acetyl-CoA C-acetyltransferase activity GO:0003985 IEA
    acetyl-CoA C-acetyltransferase activity GO:0003985 ISO
    acetyl-CoA hydrolase activity GO:0003986 IEA
    acetyl-CoA C-acyltransferase activity GO:0003988 IDA
    acetyl-CoA C-acyltransferase activity GO:0003988 IEA
    acetyl-CoA C-acyltransferase activity GO:0003988 ISO
    fatty acyl-CoA hydrolase activity GO:0047617 IDA
    fatty acyl-CoA hydrolase activity GO:0047617 IEA
    fatty acyl-CoA hydrolase activity GO:0047617 ISO
Biological Process
    acetyl-CoA metabolic process GO:0006084 IDA
    fatty acid beta-oxidation GO:0006635 IBA
    fatty acid beta-oxidation GO:0006635 IDA
    fatty acid beta-oxidation GO:0006635 IEA
    cellular response to hypoxia GO:0071456 IEA
    cellular response to hypoxia GO:0071456 ISO
    negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway GO:1901029 IEA
    negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway GO:1901029 ISO
    negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway GO:1901029 ISS
    negative regulation of mitochondrial membrane permeability involved in apoptotic process GO:1902109 IEA
    negative regulation of mitochondrial membrane permeability involved in apoptotic process GO:1902109 ISO
Subcellular Localization
    cytoplasm GO:0005737 IEA
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IEA
    mitochondrion GO:0005739 ISO
    mitochondrial matrix GO:0005759 IDA
    mitochondrial matrix GO:0005759 NAS
    intracellular membrane-bounded organelle GO:0043231 IEA
 Experiment description of studies that identified Acaa2 in sEVs
1
Experiment ID 225
MISEV standards
EM
Biophysical techniques
GAPDH|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 35
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|CD81|CD63
Enriched markers
HSPA5
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
3
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Acaa2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Acaa2 is involved
PathwayEvidenceSource
Fatty acid metabolism IEA Reactome
Metabolism IEA Reactome
Metabolism of lipids IEA Reactome
Mitochondrial Fatty Acid Beta-Oxidation IEA Reactome





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