Gene description for Dnph1
Gene name 2'-deoxynucleoside 5'-phosphate N-hydrolase 1
Gene symbol Dnph1
Other names/aliases C6orf108
RGD620382
Rcl
Species Rattus norvegicus
 Database cross references - Dnph1
ExoCarta ExoCarta_171047
Vesiclepedia VP_171047
Entrez Gene 171047
UniProt O35820  
 Dnph1 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Dnph1
Molecular Function
    identical protein binding GO:0042802 ISO
    protein homodimerization activity GO:0042803 IDA
    5-hydroxymethyl-dUMP N-hydrolase activity GO:0070694 IBA
    5-hydroxymethyl-dUMP N-hydrolase activity GO:0070694 IDA
    5-hydroxymethyl-dUMP N-hydrolase activity GO:0070694 ISO
    5-hydroxymethyl-dUMP N-hydrolase activity GO:0070694 ISS
Biological Process
    allantoin metabolic process GO:0000255 IDA
    deoxyribonucleoside monophosphate catabolic process GO:0009159 IBA
    deoxyribonucleoside monophosphate catabolic process GO:0009159 IDA
    deoxyribonucleoside monophosphate catabolic process GO:0009159 ISO
    positive regulation of cell growth GO:0030307 IDA
    epithelial cell differentiation GO:0030855 ISO
    nucleoside salvage GO:0043174 ISO
    nucleoside salvage GO:0043174 ISS
    dGMP catabolic process GO:0046055 IDA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
 Experiment description of studies that identified Dnph1 in sEVs
1
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Dnph1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Dnph1 is involved
PathwayEvidenceSource
Metabolism IEA Reactome
Metabolism of nucleotides IEA Reactome
Nucleotide catabolism IEA Reactome
Purine catabolism IEA Reactome





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