Gene description for Dnph1
Gene name 2'-deoxynucleoside 5'-phosphate N-hydrolase 1
Gene symbol Dnph1
Other names/aliases C6orf108
RGD620382
Rcl
Species Rattus norvegicus
 Database cross references - Dnph1
ExoCarta ExoCarta_171047
Entrez Gene 171047
UniProt O35820  
 Dnph1 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Dnph1
Molecular Function
    deoxyribonucleoside 5'-monophosphate N-glycosidase activity GO:0070694 ISO
    nucleoside deoxyribosyltransferase activity GO:0050144 IEA
    protein homodimerization activity GO:0042803 IDA
Biological Process
    positive regulation of cell growth GO:0030307 IDA
    nucleoside metabolic process GO:0009116 IEA
    epithelial cell differentiation GO:0030855 ISO
    deoxyribonucleoside monophosphate catabolic process GO:0009159 ISO
    nucleotide metabolic process GO:0009117 IEA
Subcellular Localization
    cytoplasm GO:0005737 ISO
    extracellular exosome GO:0070062 ISO
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 ISO
 Experiment description of studies that identified Dnph1 in exosomes
1
Experiment ID 97
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Dnph1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Dnph1 is involved
No pathways found





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