Gene description for Hadhb
Gene name hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase (trifunctional protein), beta subunit
Gene symbol Hadhb
Other names/aliases TP-beta
Species Rattus norvegicus
 Database cross references - Hadhb
ExoCarta ExoCarta_171155
Vesiclepedia VP_171155
Entrez Gene 171155
UniProt Q60587  
 Hadhb identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Hadhb
Molecular Function
    acetyl-CoA C-acetyltransferase activity GO:0003985 IBA
    acetyl-CoA C-acetyltransferase activity GO:0003985 IEA
    acetyl-CoA C-acetyltransferase activity GO:0003985 ISO
    acetyl-CoA C-acyltransferase activity GO:0003988 IDA
    acetyl-CoA C-acyltransferase activity GO:0003988 IEA
    acetyl-CoA C-acyltransferase activity GO:0003988 ISO
    protein-containing complex binding GO:0044877 IMP
    acetyl-CoA C-myristoyltransferase activity GO:0050633 IEA
    lncRNA binding GO:0106222 IEA
    lncRNA binding GO:0106222 ISO
Biological Process
    fatty acid beta-oxidation GO:0006635 IBA
    fatty acid beta-oxidation GO:0006635 IDA
    fatty acid beta-oxidation GO:0006635 IEA
    fatty acid beta-oxidation GO:0006635 ISO
    gene expression GO:0010467 IEA
    gene expression GO:0010467 ISO
    cellular response to lipopolysaccharide GO:0071222 IEA
    cellular response to lipopolysaccharide GO:0071222 ISO
Subcellular Localization
    cytoplasm GO:0005737 IEA
    mitochondrion GO:0005739 ISO
    mitochondrion GO:0005739 ISO
    mitochondrial outer membrane GO:0005741 IEA
    mitochondrial outer membrane GO:0005741 ISO
    mitochondrial outer membrane GO:0005741 ISS
    mitochondrial inner membrane GO:0005743 IEA
    mitochondrial inner membrane GO:0005743 ISO
    mitochondrial inner membrane GO:0005743 ISS
    endoplasmic reticulum GO:0005783 IEA
    endoplasmic reticulum GO:0005783 ISO
    endoplasmic reticulum GO:0005783 ISS
    mitochondrial fatty acid beta-oxidation multienzyme complex GO:0016507 IBA
    mitochondrial fatty acid beta-oxidation multienzyme complex GO:0016507 IDA
    mitochondrial fatty acid beta-oxidation multienzyme complex GO:0016507 IEA
    mitochondrial fatty acid beta-oxidation multienzyme complex GO:0016507 ISO
    mitochondrial nucleoid GO:0042645 IEA
    mitochondrial nucleoid GO:0042645 ISO
    intracellular membrane-bounded organelle GO:0043231 IEA
 Experiment description of studies that identified Hadhb in sEVs
1
Experiment ID 225
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Hadhb
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fbxo21  
Affinity Capture-MS Rattus norvegicus
2 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
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