Gene description for Hadhb
Gene name hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase (trifunctional protein), beta subunit
Gene symbol Hadhb
Other names/aliases TP-beta
Species Rattus norvegicus
 Database cross references - Hadhb
ExoCarta ExoCarta_171155
Entrez Gene 171155
UniProt Q60587  
 Hadhb identified in exosomes derived from the following tissue/cell type
Adipocytes 25998041    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Hadhb
Molecular Function
    NAD binding GO:0051287 IDA
    acetyl-CoA C-acyltransferase activity GO:0003988 ISO
    fatty-acyl-CoA binding GO:0000062 IDA
    enoyl-CoA hydratase activity GO:0004300 IDA
    poly(A) RNA binding GO:0044822 ISO
    long-chain-3-hydroxyacyl-CoA dehydrogenase activity GO:0016509 ISO
    protein complex binding GO:0032403 IMP
    3-hydroxyacyl-CoA dehydrogenase activity GO:0003857 IDA
    long-chain-enoyl-CoA hydratase activity GO:0016508 IDA
Biological Process
    fatty acid beta-oxidation GO:0006635 IEA
    oxidation-reduction process GO:0055114 ISO
Subcellular Localization
    extracellular exosome GO:0070062 ISO
    endoplasmic reticulum GO:0005783 ISS
    mitochondrial fatty acid beta-oxidation multienzyme complex GO:0016507 IDA
    mitochondrial nucleoid GO:0042645 ISO
    mitochondrial outer membrane GO:0005741 ISS
    mitochondrion GO:0005739 ISO
    mitochondrial inner membrane GO:0005743 ISS
 Experiment description of studies that identified Hadhb in exosomes
1
Experiment ID 225
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors Lee JE, Moon PG, Lee IK, Baek MC
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 97
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Hadhb
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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