Gene description for Atg3
Gene name autophagy related 3
Gene symbol Atg3
Other names/aliases Apg3l
PIG-1
Pig1
Species Rattus norvegicus
 Database cross references - Atg3
ExoCarta ExoCarta_171415
Vesiclepedia VP_171415
Entrez Gene 171415
UniProt Q6AZ50  
 Atg3 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Atg3
Molecular Function
    Atg8-family ligase activity GO:0019776 IEA
    Atg8-family ligase activity GO:0019776 ISO
    Atg8-family ligase activity GO:0019776 ISS
    Atg12 transferase activity GO:0019777 IEA
    Atg12 transferase activity GO:0019777 ISO
    Atg12 transferase activity GO:0019777 ISS
    ubiquitin-like protein transferase activity GO:0019787 IEA
    ubiquitin-like protein transferase activity GO:0019787 ISO
    enzyme binding GO:0019899 IEA
    enzyme binding GO:0019899 ISO
    Atg8-family conjugating enzyme activity GO:0141046 IBA
    Atg8-family conjugating enzyme activity GO:0141046 IEA
    Atg8-family conjugating enzyme activity GO:0141046 IMP
    Atg8-family conjugating enzyme activity GO:0141046 ISO
    Atg8-family conjugating enzyme activity GO:0141046 ISS
Biological Process
    autophagosome assembly GO:0000045 IBA
    autophagosome assembly GO:0000045 IEA
    autophagosome assembly GO:0000045 ISO
    autophagosome assembly GO:0000045 ISO
    autophagosome assembly GO:0000045 ISS
    autophagy of mitochondrion GO:0000422 IBA
    autophagy GO:0006914 IEA
    autophagy GO:0006914 IEP
    autophagy GO:0006914 ISO
    autophagy GO:0006914 ISS
    protein transport GO:0015031 IEA
    macroautophagy GO:0016236 ISO
    protein ubiquitination GO:0016567 IEA
    protein ubiquitination GO:0016567 ISO
    mitochondrial fragmentation involved in apoptotic process GO:0043653 IEA
    mitochondrial fragmentation involved in apoptotic process GO:0043653 ISO
    mitochondrial fragmentation involved in apoptotic process GO:0043653 ISS
    nucleophagy GO:0044804 IBA
    negative regulation of phagocytosis GO:0050765 IEA
    negative regulation of phagocytosis GO:0050765 ISO
    glycophagy GO:0061723 IBA
    regulation of cilium assembly GO:1902017 IEA
    regulation of cilium assembly GO:1902017 ISO
Subcellular Localization
    phagophore assembly site GO:0000407 IBA
    cytoplasm GO:0005737 IEA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    Atg12-Atg5-Atg16 complex GO:0034274 IEA
    Atg12-Atg5-Atg16 complex GO:0034274 ISO
 Experiment description of studies that identified Atg3 in sEVs
1
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Atg3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Atg12  
Affinity Capture-Western Rattus norvegicus
View the network image/svg+xml
 Pathways in which Atg3 is involved
PathwayEvidenceSource
Autophagy IEA Reactome
Macroautophagy IEA Reactome





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