Gene description for Atg3
Gene name autophagy related 3
Gene symbol Atg3
Other names/aliases Apg3l
PIG-1
Pig1
Species Rattus norvegicus
 Database cross references - Atg3
ExoCarta ExoCarta_171415
Entrez Gene 171415
UniProt Q6AZ50  
 Atg3 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Atg3
Molecular Function
    ubiquitin-like protein transferase activity GO:0019787 ISO
    molecular_function GO:0003674 ND
    Atg8 ligase activity GO:0019776 ISS
    enzyme binding GO:0019899 ISO
    ligase activity GO:0016874 IEA
    Atg12 transferase activity GO:0019777 ISS
Biological Process
    mitochondrion degradation GO:0000422 IBA
    autophagy GO:0006914 IEP
    protein ubiquitination GO:0016567 ISO
    regulation of cilium assembly GO:1902017 ISO
    protein transport GO:0015031 IEA
    autophagosome assembly GO:0000045 ISS
    mitochondrial fragmentation involved in apoptotic process GO:0043653 ISS
    nucleophagy GO:0044804 IBA
    cellular protein modification process GO:0006464 ISO
Subcellular Localization
    cytoplasmic ubiquitin ligase complex GO:0000153 ISO
    cytosol GO:0005829 ISO
 Experiment description of studies that identified Atg3 in exosomes
1
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Atg3
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Atg3 is involved
No pathways found





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