Gene description for Man2a1
Gene name mannosidase 2, alpha 1
Gene symbol Man2a1
Other names/aliases Mana-2
Mana2
Map-2
Species Mus musculus
 Database cross references - Man2a1
ExoCarta ExoCarta_17158
Vesiclepedia VP_17158
Entrez Gene 17158
UniProt P27046  
 Man2a1 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Man2a1
Molecular Function
    hydrolase activity, hydrolyzing O-glycosyl compounds GO:0004553 IEA
    alpha-mannosidase activity GO:0004559 IEA
    catalytic activity GO:0003824 IEA
    carbohydrate binding GO:0030246 IEA
    metal ion binding GO:0046872 IEA
    hydrolase activity, acting on glycosyl bonds GO:0016798 IEA
    hydrolase activity, hydrolyzing N-glycosyl compounds GO:0016799 IDA
    mannosidase activity GO:0015923 IDA
    hydrolase activity GO:0016787 IEA
    mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase activity GO:0004572 IEA
    zinc ion binding GO:0008270 IEA
Biological Process
    lung alveolus development GO:0048286 IGI
    mitochondrion organization GO:0007005 IGI
    retina morphogenesis in camera-type eye GO:0060042 IMP
    vacuole organization GO:0007033 IGI
    N-glycan processing GO:0006491 IGI
    mannose metabolic process GO:0006013 IEA
    respiratory gaseous exchange GO:0007585 IGI
    in utero embryonic development GO:0001701 IGI
    metabolic process GO:0008152 IEA
    liver development GO:0001889 IGI
    carbohydrate metabolic process GO:0005975 IEA
    positive regulation of neurogenesis GO:0050769 IMP
Subcellular Localization
    integral component of membrane GO:0016021 IEA
    Golgi apparatus GO:0005794 IDA
    membrane GO:0016020 ISO
    Golgi membrane GO:0000139 IDA
    extracellular exosome GO:0070062 ISO
    cis-Golgi network GO:0005801 IDA
 Experiment description of studies that identified Man2a1 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Man2a1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Man2a1 is involved
PathwayEvidenceSource
Reactions specific to the complex N-glycan synthesis pathway IEA Reactome





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