Gene description for Man2a1
Gene name mannosidase 2, alpha 1
Gene symbol Man2a1
Other names/aliases Mana-2
Mana2
Map-2
Species Mus musculus
 Database cross references - Man2a1
ExoCarta ExoCarta_17158
Vesiclepedia VP_17158
Entrez Gene 17158
UniProt P27046  
 Man2a1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Man2a1
Molecular Function
    alpha-mannosidase activity GO:0004559 IBA
    mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase activity GO:0004572 IEA
    mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase activity GO:0004572 ISO
    mannosidase activity GO:0015923 IDA
    hydrolase activity GO:0016787 IDA
    hydrolase activity, hydrolyzing N-glycosyl compounds GO:0016799 IDA
    carbohydrate binding GO:0030246 IEA
    identical protein binding GO:0042802 ISO
    protein homodimerization activity GO:0042803 IEA
    protein homodimerization activity GO:0042803 ISO
    metal ion binding GO:0046872 IEA
Biological Process
    in utero embryonic development GO:0001701 IGI
    liver development GO:0001889 IGI
    mannose metabolic process GO:0006013 IEA
    protein glycosylation GO:0006486 IEA
    N-glycan processing GO:0006491 IBA
    N-glycan processing GO:0006491 IGI
    N-glycan processing GO:0006491 ISO
    mitochondrion organization GO:0007005 IGI
    vacuole organization GO:0007033 IGI
    respiratory gaseous exchange by respiratory system GO:0007585 IGI
    lung alveolus development GO:0048286 IGI
    positive regulation of neurogenesis GO:0050769 IMP
    retina morphogenesis in camera-type eye GO:0060042 IMP
Subcellular Localization
    Golgi membrane GO:0000139 IBA
    Golgi membrane GO:0000139 IDA
    Golgi membrane GO:0000139 ISO
    extracellular space GO:0005615 IDA
    Golgi apparatus GO:0005794 IDA
    Golgi medial cisterna GO:0005797 IEA
    Golgi medial cisterna GO:0005797 ISO
    cis-Golgi network GO:0005801 IDA
 Experiment description of studies that identified Man2a1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Man2a1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here