Gene description for Man2b1
Gene name mannosidase 2, alpha B1
Gene symbol Man2b1
Other names/aliases AW107687
LAMAN
Species Mus musculus
 Database cross references - Man2b1
ExoCarta ExoCarta_17159
Vesiclepedia VP_17159
Entrez Gene 17159
UniProt O09159  
 Man2b1 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Man2b1
Molecular Function
    zinc ion binding GO:0008270 IEA
    beta-6-sulfate-N-acetylglucosaminidase activity GO:0052769 IEA
    starch alpha-glucosidase activity GO:0044654 IEA
    carbohydrate binding GO:0030246 IEA
    mannosyl-oligosaccharide mannosidase activity GO:0015924 IEA
    lytic transglycosylase activity GO:0008933 IEA
    trehalase activity GO:0015927 IEA
    alpha-mannosidase activity GO:0004559 ISO
    galactosidase activity GO:0015925 IEA
    metal ion binding GO:0046872 IEA
    fucosidase activity GO:0015928 IEA
    glucosidase activity GO:0015926 IEA
    hydrolase activity, acting on glycosyl bonds GO:0016798 IEA
    catalytic activity GO:0003824 IEA
    amylase activity GO:0016160 IEA
    glucan endo-1,4-beta-glucosidase activity GO:0052859 IEA
    mannosidase activity GO:0015923 IEA
    beta-glucanase activity GO:0052736 IEA
    hexosaminidase activity GO:0015929 IEA
    hydrolase activity GO:0016787 IEA
    mannose binding GO:0005537 ISO
    dextrin alpha-glucosidase activity GO:0044653 IEA
    hydrolase activity, hydrolyzing O-glycosyl compounds GO:0004553 IEA
Biological Process
    carbohydrate metabolic process GO:0005975 IEA
    cellular protein modification process GO:0006464 ISO
    metabolic process GO:0008152 IEA
    mannose metabolic process GO:0006013 IMP
    learning or memory GO:0007611 IMP
Subcellular Localization
    extracellular exosome GO:0070062 ISO
    lysosome GO:0005764 ISO
 Experiment description of studies that identified Man2b1 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Man2b1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Man2b1 is involved
No pathways found





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