Gene description for Masp1
Gene name mannan-binding lectin serine peptidase 1
Gene symbol Masp1
Other names/aliases AW048060
CCPII
Crarf
Masp1/3
Species Mus musculus
 Database cross references - Masp1
ExoCarta ExoCarta_17174
Vesiclepedia VP_17174
Entrez Gene 17174
UniProt P98064  
 Masp1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Masp1
Molecular Function
    serine-type endopeptidase activity GO:0004252 IBA
    serine-type endopeptidase activity GO:0004252 IDA
    serine-type endopeptidase activity GO:0004252 ISO
    calcium ion binding GO:0005509 ISO
    calcium ion binding GO:0005509 ISS
    peptidase activity GO:0008233 ISO
    identical protein binding GO:0042802 ISO
    protein homodimerization activity GO:0042803 ISO
    protein homodimerization activity GO:0042803 ISS
    calcium-dependent protein binding GO:0048306 IEA
    calcium-dependent protein binding GO:0048306 ISO
Biological Process
    complement activation, lectin pathway GO:0001867 IBA
    complement activation, lectin pathway GO:0001867 IMP
    complement activation, lectin pathway GO:0001867 ISO
    proteolysis GO:0006508 IEA
    negative regulation of complement activation GO:0045916 ISO
Subcellular Localization
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    extracellular space GO:0005615 ISO
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
 Experiment description of studies that identified Masp1 in sEVs
1
Experiment ID 907
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
EM
Biophysical techniques
CD81|FLOT1
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Masp1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Thrb  
Affinity Capture-MS Mus musculus
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