Gene description for Mcm5
Gene name minichromosome maintenance deficient 5, cell division cycle 46 (S. cerevisiae)
Gene symbol Mcm5
Other names/aliases AA617332
AI324988
AL033333
Cdc46
Mcmd5
P1-CDC46
Species Mus musculus
 Database cross references - Mcm5
ExoCarta ExoCarta_17218
Vesiclepedia VP_17218
Entrez Gene 17218
 Mcm5 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Mcm5
Molecular Function
    DNA replication origin binding GO:0003688 IEA
    DNA clamp loader activity GO:0003689 IEA
    single-stranded DNA binding GO:0003697 IBA
    ATP binding GO:0005524 IEA
    four-way junction helicase activity GO:0009378 IEA
    ATP hydrolysis activity GO:0016887 IEA
    single-stranded DNA helicase activity GO:0017116 IBA
    double-stranded DNA helicase activity GO:0036121 IEA
    3'-5' DNA helicase activity GO:0043138 IBA
    forked DNA-dependent helicase activity GO:0061749 IEA
    cohesin loader activity GO:0061775 IEA
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
    single-stranded 3'-5' DNA helicase activity GO:1990518 IEA
Biological Process
    double-strand break repair via break-induced replication GO:0000727 IBA
    DNA unwinding involved in DNA replication GO:0006268 ISO
    DNA replication initiation GO:0006270 IBA
    DNA replication initiation GO:0006270 IEA
    premeiotic DNA replication GO:0006279 NAS
    chromatin remodeling GO:0006338 IEA
    chromatin looping GO:0140588 IEA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 NAS
    nucleoplasm GO:0005654 ISO
    cytosol GO:0005829 IEA
    MCM complex GO:0042555 IBA
    MCM complex GO:0042555 ISO
    MCM complex GO:0042555 ISS
    CMG complex GO:0071162 ISO
    CMG complex GO:0071162 ISS
 Experiment description of studies that identified Mcm5 in sEVs
1
Experiment ID 907
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Mcm5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fancd2  
Affinity Capture-MS Mus musculus
2 Atxn1  
Affinity Capture-MS Mus musculus
3 Eed  
Affinity Capture-MS Mus musculus
4 Atg16l1  
Affinity Capture-MS Mus musculus
5 Pou5f1  
Affinity Capture-MS Mus musculus
View the network image/svg+xml



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