Gene description for Mdfi
Gene name MyoD family inhibitor
Gene symbol Mdfi
Other names/aliases I-mf
I-mfa
Species Mus musculus
 Database cross references - Mdfi
ExoCarta ExoCarta_17240
Vesiclepedia VP_17240
Entrez Gene 17240
UniProt P70331  
 Mdfi identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Mdfi
Molecular Function
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    DNA-binding transcription factor binding GO:0140297 IPI
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IMP
    dorsal/ventral axis specification GO:0009950 IMP
    regulation of Wnt signaling pathway GO:0030111 IBA
    regulation of Wnt signaling pathway GO:0030111 IDA
    negative regulation of Wnt signaling pathway GO:0030178 IGI
    negative regulation of DNA binding GO:0043392 IDA
    negative regulation of DNA-templated transcription GO:0045892 IBA
    regulation of JNK cascade GO:0046328 IBA
    regulation of JNK cascade GO:0046328 IDA
    embryonic skeletal system morphogenesis GO:0048704 IMP
    trophoblast giant cell differentiation GO:0060707 IMP
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IEA
 Experiment description of studies that identified Mdfi in sEVs
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Mdfi
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Zic2  
Affinity Capture-Western Mus musculus
2 TCF7L1  
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which Mdfi is involved
No pathways found





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