Gene description for Mid1
Gene name midline 1
Gene symbol Mid1
Other names/aliases 61B3-R
DXHXS1141
Fxy
Trim18
Species Mus musculus
 Database cross references - Mid1
ExoCarta ExoCarta_17318
Vesiclepedia VP_17318
Entrez Gene 17318
UniProt O70583  
 Mid1 identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Mid1
Molecular Function
    protein binding GO:0005515 IPI
    microtubule binding GO:0008017 IEA
    microtubule binding GO:0008017 ISO
    zinc ion binding GO:0008270 IEA
    transferase activity GO:0016740 IEA
    enzyme binding GO:0019899 ISO
    ubiquitin protein ligase binding GO:0031625 IEA
    ubiquitin protein ligase binding GO:0031625 ISO
    identical protein binding GO:0042802 ISO
    protein homodimerization activity GO:0042803 IEA
    protein homodimerization activity GO:0042803 ISO
    phosphoprotein binding GO:0051219 IEA
    phosphoprotein binding GO:0051219 ISO
Biological Process
    negative regulation of microtubule depolymerization GO:0007026 IGI
    positive regulation of stress-activated MAPK cascade GO:0032874 IEA
    positive regulation of stress-activated MAPK cascade GO:0032874 ISO
    protein localization to microtubule GO:0035372 IEA
    protein localization to microtubule GO:0035372 ISO
    regulation of microtubule cytoskeleton organization GO:0070507 IBA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    Golgi apparatus GO:0005794 IEA
    Golgi apparatus GO:0005794 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    microtubule GO:0005874 ISO
    cytoplasmic microtubule GO:0005881 IEA
    cytoplasmic microtubule GO:0005881 ISO
    microtubule cytoskeleton GO:0015630 IDA
    centriolar satellite GO:0034451 IEA
    centriolar satellite GO:0034451 ISO
 Experiment description of studies that identified Mid1 in sEVs
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Mid1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Mid1ip1  
Affinity Capture-Western Mus musculus
Two-hybrid Mus musculus
Affinity Capture-Western Mus musculus
Co-localization Mus musculus
2 Igbp1  
Two-hybrid Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
3 Kcna3  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Mid1 is involved
No pathways found





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