Gene description for Mif
Gene name macrophage migration inhibitory factor
Gene symbol Mif
Other names/aliases GIF
Glif
Species Mus musculus
 Database cross references - Mif
ExoCarta ExoCarta_17319
Entrez Gene 17319
UniProt P34884  
 Mif identified in exosomes derived from the following tissue/cell type
Neural stem cells 25242146    
 Gene ontology annotations for Mif
Molecular Function
    chemoattractant activity GO:0042056 ISO
    cytokine activity GO:0005125 ISO
    protein complex binding GO:0032403 ISO
    phenylpyruvate tautomerase activity GO:0050178 ISO
    dopachrome isomerase activity GO:0004167 ISO
    receptor binding GO:0005102 ISO
    isomerase activity GO:0016853 IEA
    cytokine receptor binding GO:0005126 ISO
Biological Process
    prostaglandin biosynthetic process GO:0001516 ISO
    carboxylic acid metabolic process GO:0019752 ISO
    positive regulation of potassium ion transport GO:0043268 ISO
    positive regulation of ERK1 and ERK2 cascade GO:0070374 ISO
    cell aging GO:0007569 IMP
    negative regulation of mature B cell apoptotic process GO:0002906 IDA
    negative regulation of myeloid cell apoptotic process GO:0033033 IMP
    positive regulation of prostaglandin secretion involved in immune response GO:0061078 IMP
    positive regulation of adaptive immune response GO:0002821 NAS
    positive regulation of lipopolysaccharide-mediated signaling pathway GO:0031666 IMP
    negative regulation of DNA damage response, signal transduction by p53 class mediator GO:0043518 ISO
    positive regulation of glycolytic process GO:0045821 ISO
    negative regulation of cell cycle arrest GO:0071157 ISO
    positive regulation of immune response GO:0050778 ISO
    drinking behavior GO:0042756 ISO
    brain renin-angiotensin system GO:0002035 ISO
    negative regulation of cellular protein metabolic process GO:0032269 IDA
    cell proliferation GO:0008283 ISO
    negative regulation of apoptotic process GO:0043066 ISO
    protein homotrimerization GO:0070207 ISO
    positive regulation of cytokine secretion GO:0050715 ISO
    positive regulation of myeloid leukocyte cytokine production involved in immune response GO:0061081 IMP
    positive regulation of chemokine (C-X-C motif) ligand 2 production GO:2000343 IDA
    inflammatory response GO:0006954 IEA
    cell surface receptor signaling pathway GO:0007166 ISO
    innate immune response GO:0045087 IEA
    positive regulation of arachidonic acid secretion GO:0090238 IDA
    DNA damage response, signal transduction by p53 class mediator GO:0030330 IMP
    immune system process GO:0002376 IEA
    positive regulation of protein phosphorylation GO:0001934 IDA
    positive regulation of fibroblast proliferation GO:0048146 ISO
    positive regulation of phosphorylation GO:0042327 ISO
    positive regulation of peptidyl-serine phosphorylation GO:0033138 ISO
    positive regulation of protein kinase A signaling GO:0010739 ISO
    negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator GO:1902166 ISO
    positive regulation of B cell proliferation GO:0030890 ISO
    positive regulation of axon regeneration GO:0048680 ISO
    positive regulation of glucose import GO:0046326 ISO
    positive regulation of MAP kinase activity GO:0043406 IDA
    regulation of cell proliferation GO:0042127 IMP
    positive regulation of smooth muscle cell migration GO:0014911 ISO
    positive regulation of acute inflammatory response GO:0002675 ISO
    spermatogenesis GO:0007283 ISO
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 ISO
    negative regulation of gene expression GO:0010629 ISO
    negative regulation of cell aging GO:0090344 ISO
    positive chemotaxis GO:0050918 ISO
Subcellular Localization
    vesicle GO:0031982 ISO
    extracellular space GO:0005615 ISO
    cytoplasm GO:0005737 ISO
    extracellular exosome GO:0070062 ISO
    myelin sheath GO:0043209 IDA
    cell surface GO:0009986 ISO
    nucleus GO:0005634 ISO
    intracellular GO:0005622 IMP
    nucleoplasm GO:0005654 ISO
    extracellular region GO:0005576 ISO
 Experiment description of studies that identified Mif in exosomes
1
Experiment ID 263
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-γ via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th1 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Mif
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Mif is involved
No pathways found





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