Gene description for Mif
Gene name macrophage migration inhibitory factor
Gene symbol Mif
Other names/aliases GIF
Glif
Species Mus musculus
 Database cross references - Mif
ExoCarta ExoCarta_17319
Entrez Gene 17319
UniProt P34884  
 Mif identified in exosomes derived from the following tissue/cell type
Neural stem cells 25242146    
 Gene ontology annotations for Mif
Molecular Function
    chemoattractant activity GO:0042056 ISO
    cytokine activity GO:0005125 ISO
    protein complex binding GO:0032403 ISO
    phenylpyruvate tautomerase activity GO:0050178 ISO
    dopachrome isomerase activity GO:0004167 ISO
    receptor binding GO:0005102 ISO
    isomerase activity GO:0016853 IEA
    cytokine receptor binding GO:0005126 ISO
Biological Process
    prostaglandin biosynthetic process GO:0001516 ISO
    carboxylic acid metabolic process GO:0019752 ISO
    positive regulation of potassium ion transport GO:0043268 ISO
    positive regulation of ERK1 and ERK2 cascade GO:0070374 ISO
    cell aging GO:0007569 IMP
    negative regulation of mature B cell apoptotic process GO:0002906 IDA
    negative regulation of myeloid cell apoptotic process GO:0033033 IMP
    positive regulation of prostaglandin secretion involved in immune response GO:0061078 IMP
    positive regulation of adaptive immune response GO:0002821 NAS
    positive regulation of lipopolysaccharide-mediated signaling pathway GO:0031666 IMP
    negative regulation of DNA damage response, signal transduction by p53 class mediator GO:0043518 ISO
    positive regulation of glycolytic process GO:0045821 ISO
    negative regulation of cell cycle arrest GO:0071157 ISO
    positive regulation of immune response GO:0050778 ISO
    drinking behavior GO:0042756 ISO
    brain renin-angiotensin system GO:0002035 ISO
    negative regulation of cellular protein metabolic process GO:0032269 IDA
    cell proliferation GO:0008283 ISO
    negative regulation of apoptotic process GO:0043066 ISO
    protein homotrimerization GO:0070207 ISO
    positive regulation of cytokine secretion GO:0050715 ISO
    positive regulation of myeloid leukocyte cytokine production involved in immune response GO:0061081 IMP
    positive regulation of chemokine (C-X-C motif) ligand 2 production GO:2000343 IDA
    inflammatory response GO:0006954 IEA
    cell surface receptor signaling pathway GO:0007166 ISO
    innate immune response GO:0045087 IEA
    positive regulation of arachidonic acid secretion GO:0090238 IDA
    DNA damage response, signal transduction by p53 class mediator GO:0030330 IMP
    immune system process GO:0002376 IEA
    positive regulation of protein phosphorylation GO:0001934 IDA
    positive regulation of fibroblast proliferation GO:0048146 ISO
    positive regulation of phosphorylation GO:0042327 ISO
    positive regulation of peptidyl-serine phosphorylation GO:0033138 ISO
    positive regulation of protein kinase A signaling GO:0010739 ISO
    negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator GO:1902166 ISO
    positive regulation of B cell proliferation GO:0030890 ISO
    positive regulation of axon regeneration GO:0048680 ISO
    positive regulation of glucose import GO:0046326 ISO
    positive regulation of MAP kinase activity GO:0043406 IDA
    regulation of cell proliferation GO:0042127 IMP
    positive regulation of smooth muscle cell migration GO:0014911 ISO
    positive regulation of acute inflammatory response GO:0002675 ISO
    spermatogenesis GO:0007283 ISO
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 ISO
    negative regulation of gene expression GO:0010629 ISO
    negative regulation of cell aging GO:0090344 ISO
    positive chemotaxis GO:0050918 ISO
Subcellular Localization
    vesicle GO:0031982 ISO
    extracellular space GO:0005615 ISO
    cytoplasm GO:0005737 ISO
    extracellular exosome GO:0070062 ISO
    myelin sheath GO:0043209 IDA
    cell surface GO:0009986 ISO
    nucleus GO:0005634 ISO
    intracellular GO:0005622 IMP
    nucleoplasm GO:0005654 ISO
    extracellular region GO:0005576 ISO
 Experiment description of studies that identified Mif in exosomes
1
Experiment ID 263
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
CD63|CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-γ via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th1 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Mif
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Mif is involved
No pathways found





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