Experiment description of studies that identified Marcksl1 in exosomes |
1 |
Experiment ID |
214 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✘
|
EV Cytosolic markers |
✘
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23658846
|
Organism |
Mus musculus |
Experiment description |
Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis. |
Authors |
Hassani K, Olivier M. |
Journal name |
PLoS Negl Trop Dis
|
Publication year |
2013 |
Sample |
Macrophages |
Sample name |
Leishmania-infected-Macrophage (J774A.1) |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Filtration Protease inhibitors Sucrose density gradient Filtration Ultracentrifugation |
Flotation density |
1.13-1.19 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
2 |
Experiment ID |
215 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✘
|
EV Cytosolic markers |
✘
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23658846
|
Organism |
Mus musculus |
Experiment description |
Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis. |
Authors |
Hassani K, Olivier M. |
Journal name |
PLoS Negl Trop Dis
|
Publication year |
2013 |
Sample |
Macrophages |
Sample name |
LPS-treated-Macrophage (J774A.1) |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Filtration Protease inhibitors Sucrose density gradient Filtration Ultracentrifugation |
Flotation density |
1.13-1.19 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
3 |
Experiment ID |
216 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✘
|
EV Cytosolic markers |
✘
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23658846
|
Organism |
Mus musculus |
Experiment description |
Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis. |
Authors |
Hassani K, Olivier M. |
Journal name |
PLoS Negl Trop Dis
|
Publication year |
2013 |
Sample |
Macrophages |
Sample name |
Normal-Macrophage (J774A.1) |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Filtration Protease inhibitors Sucrose density gradient Filtration Ultracentrifugation |
Flotation density |
1.13-1.19 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
4 |
Experiment ID |
33 |
ISEV standards |
✔
EM|IEM
|
EV Biophysical techniques |
✔
Alix|TSG101
|
EV Cytosolic markers |
✘
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
21136642
|
Organism |
Mus musculus |
Experiment description |
Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons? |
Authors |
Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J |
Journal name |
PROTEOMICS_CL
|
Publication year |
2007 |
Sample |
Oligodendrocytes |
Sample name |
Oligodendrocytes Oli-neu |
Isolation/purification methods |
Differential centrifugation Sucrose density gradient |
Flotation density |
1.10-1.14 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [QTOF] Western blotting Immunoelectron Microscopy |
|
|