Experiment description of studies that identified Mug1 in sEVs

1 Experiment ID 303 MISEV standards ✔ EM Biophysical techniques ✔ Cd63|Cd81|Cd9 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33431899     Organism Mus musculus Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG." Journal name Nat Commun Publication year 2021 Sample Faeces Sample name Intestinal epithelial cells - C57BL/6 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient centrifugation Flotation density - Molecules identified in the study Protein Lipid miRNA Methods used in the study Western blotting Mass spectrometry Immunofluorescence miRNA array HPLC

2 Experiment ID 304 MISEV standards ✔ EM Biophysical techniques ✔ Cd63|Cd81|Cd9 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33431899     Organism Mus musculus Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG." Journal name Nat Commun Publication year 2021 Sample Faeces Sample name Intestinal epithelial cells - C57BL/6 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient centrifugation Flotation density - Molecules identified in the study Protein Lipid miRNA Methods used in the study Western blotting Mass spectrometry Immunofluorescence miRNA array HPLC

3 Experiment ID 15 MISEV standards ✔ EM Biophysical techniques ✔ CD63 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 17486113     Organism Mus musculus Homo sapiens Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO" Journal name NCB Publication year 2007 Sample Mast cells Sample name MC9 Bone marrow-derived mast cells HMC-1 Isolation/purification methods Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.11-1.21 g/mL Molecules identified in the study Protein mRNA miRNA Methods used in the study Mass spectrometry [MALDI TOF] Western blotting Microarray miRCURY LNA Array

Protein-protein interactions for Mug1

Protein Interactor ExoCarta ID Identification method PubMed Species 1 Klk8   Reconstituted Complex Mus musculus 2 Fancd2   Affinity Capture-MS Mus musculus View the network

Pathways in which Mug1 is involved

No pathways found