Gene description for Usp34
Gene name ubiquitin specific peptidase 34
Gene symbol Usp34
Other names/aliases A530081C03Rik
Murr2
Species Mus musculus
 Database cross references - Usp34
ExoCarta ExoCarta_17847
Vesiclepedia VP_17847
Entrez Gene 17847
UniProt Q6ZQ93  
 Usp34 identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Usp34
Molecular Function
    cysteine-type endopeptidase activity GO:0004197 ISO
    cysteine-type endopeptidase activity GO:0004197 ISS
    cysteine-type deubiquitinase activity GO:0004843 IBA
    cysteine-type deubiquitinase activity GO:0004843 ISO
    cysteine-type deubiquitinase activity GO:0004843 ISS
Biological Process
    proteolysis GO:0006508 IEA
    Wnt signaling pathway GO:0016055 IEA
    regulation of protein stability GO:0031647 IBA
    protein K48-linked deubiquitination GO:0071108 ISO
    protein K48-linked deubiquitination GO:0071108 ISS
    positive regulation of canonical Wnt signaling pathway GO:0090263 ISO
    positive regulation of canonical Wnt signaling pathway GO:0090263 ISS
Subcellular Localization
    nucleus GO:0005634 IBA
    cytosol GO:0005829 IBA
 Experiment description of studies that identified Usp34 in sEVs
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Usp34
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 A230046K03Rik  
Co-fractionation Mus musculus
2 Ubc  
Affinity Capture-MS Mus musculus
View the network image/svg+xml



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