Gene description for Myo9b
Gene name myosin IXb
Gene symbol Myo9b
Other names/aliases -
Species Mus musculus
 Database cross references - Myo9b
ExoCarta ExoCarta_17925
Vesiclepedia VP_17925
Entrez Gene 17925
 Myo9b identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Myo9b
Molecular Function
    microfilament motor activity GO:0000146 IBA
    microfilament motor activity GO:0000146 IDA
    microfilament motor activity GO:0000146 ISO
    cytoskeletal motor activity GO:0003774 ISO
    actin binding GO:0003779 ISO
    actin binding GO:0003779 ISS
    GTPase activator activity GO:0005096 IDA
    GTPase activator activity GO:0005096 IMP
    GTPase activator activity GO:0005096 ISO
    calmodulin binding GO:0005516 IEA
    calmodulin binding GO:0005516 ISO
    ATP binding GO:0005524 IBA
    ATP binding GO:0005524 ISO
    zinc ion binding GO:0008270 ISO
    ATP hydrolysis activity GO:0016887 IBA
    ATP hydrolysis activity GO:0016887 ISO
    small GTPase binding GO:0031267 ISO
    ATP-dependent protein binding GO:0043008 ISO
    ADP binding GO:0043531 ISO
    metal ion binding GO:0046872 IEA
    Roundabout binding GO:0048495 ISO
    actin filament binding GO:0051015 IBA
    actin filament binding GO:0051015 ISO
Biological Process
    monocyte chemotaxis GO:0002548 IMP
    Rho protein signal transduction GO:0007266 IMP
    Rho protein signal transduction GO:0007266 ISO
    establishment of cell polarity GO:0030010 IMP
    actin filament-based movement GO:0030048 IBA
    actin filament-based movement GO:0030048 ISO
    actin filament-based movement GO:0030048 ISS
    actin-myosin filament sliding GO:0033275 ISO
    regulation of Rho protein signal transduction GO:0035023 ISO
    regulation of Rho protein signal transduction GO:0035023 ISS
    Roundabout signaling pathway GO:0035385 ISO
    Roundabout signaling pathway GO:0035385 ISS
    positive regulation of GTPase activity GO:0043547 IDA
    positive regulation of GTPase activity GO:0043547 IMP
    macrophage chemotaxis GO:0048246 IMP
    lamellipodium morphogenesis GO:0072673 IBA
    lamellipodium morphogenesis GO:0072673 IMP
Subcellular Localization
    ruffle GO:0001726 IBA
    ruffle GO:0001726 ISO
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    cytosol GO:0005829 ISO
    actin filament GO:0005884 IBA
    actin filament GO:0005884 ISO
    cell cortex GO:0005938 ISO
    cell cortex GO:0005938 ISS
    myosin complex GO:0016459 IEA
    lamellipodium GO:0030027 IBA
    lamellipodium GO:0030027 IDA
    lamellipodium GO:0030027 ISO
    filopodium tip GO:0032433 IDA
    perinuclear region of cytoplasm GO:0048471 ISO
    perinuclear region of cytoplasm GO:0048471 ISS
    postsynaptic actin cytoskeleton GO:0098871 ISO
 Experiment description of studies that identified Myo9b in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Myo9b
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Zbtb7b  
Affinity Capture-MS Mus musculus
2 Fancd2  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Myo9b is involved
No pathways found





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