Gene description for Nme1
Gene name NME/NM23 nucleoside diphosphate kinase 1
Gene symbol Nme1
Other names/aliases AL024257
NDPK-A
NM23-M1
NM23A
Species Mus musculus
 Database cross references - Nme1
ExoCarta ExoCarta_18102
Entrez Gene 18102
UniProt P15532  
 Nme1 identified in exosomes derived from the following tissue/cell type
Neural stem cells 25242146    
Oligodendrocytes 21136642    
 Gene ontology annotations for Nme1
Molecular Function
    RNA polymerase II regulatory region sequence-specific DNA binding GO:0000977 ISO
    nucleotide binding GO:0000166 IEA
    identical protein binding GO:0042802 ISO
    GTP binding GO:0005525 ISO
    deoxyribonuclease activity GO:0004536 ISO
    ATP binding GO:0005524 ISO
    nucleoside diphosphate kinase activity GO:0004550 ISO
    kinase activity GO:0016301 IEA
    ribosomal small subunit binding GO:0043024 ISO
    intermediate filament binding GO:0019215 ISO
    enzyme binding GO:0019899 ISO
    poly(A) RNA binding GO:0044822 ISO
    metal ion binding GO:0046872 IEA
    gamma-tubulin binding GO:0043015 ISO
    transferase activity GO:0016740 IEA
    magnesium ion binding GO:0000287 ISO
    single-stranded DNA binding GO:0003697 ISO
    protein kinase binding GO:0019901 ISO
Biological Process
    cell differentiation GO:0030154 IEA
    CTP biosynthetic process GO:0006241 IEA
    pyrimidine nucleotide metabolic process GO:0006220 IBA
    nervous system development GO:0007399 IEA
    purine nucleotide metabolic process GO:0006163 IBA
    phosphorylation GO:0016310 IEA
    positive regulation of epithelial cell proliferation GO:0050679 ISO
    lactation GO:0007595 IMP
    mammary gland development GO:0030879 IMP
    nucleoside diphosphate phosphorylation GO:0006165 ISO
    positive regulation of neuron projection development GO:0010976 ISO
    endocytosis GO:0006897 IEA
    DNA metabolic process GO:0006259 ISO
    nucleoside triphosphate biosynthetic process GO:0009142 IBA
    GTP biosynthetic process GO:0006183 IEA
    negative regulation of myeloid leukocyte differentiation GO:0002762 ISO
    regulation of apoptotic process GO:0042981 IBA
    negative regulation of gene expression GO:0010629 ISO
    nucleotide metabolic process GO:0009117 IEA
    UTP biosynthetic process GO:0006228 IEA
    positive regulation of DNA binding GO:0043388 ISO
    nucleic acid phosphodiester bond hydrolysis GO:0090305 ISO
Subcellular Localization
    centrosome GO:0005813 ISO
    mitochondrial outer membrane GO:0005741 ISO
    perinuclear region of cytoplasm GO:0048471 ISO
    ruffle membrane GO:0032587 ISO
    membrane GO:0016020 ISO
    extracellular exosome GO:0070062 ISO
    intracellular GO:0005622 IBA
    cytoplasm GO:0005737 IEA
    cytosol GO:0005829 ISO
    myelin sheath GO:0043209 IDA
    nucleus GO:0005634 ISO
    intermediate filament GO:0005882 ISO
    mitochondrion GO:0005739 IDA
 Experiment description of studies that identified Nme1 in exosomes
1
Experiment ID 263
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
CD63|CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-γ via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th1 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 33
ISEV standards
EM|IEM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for Nme1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Nme1 is involved
PathwayEvidenceSource
Synthesis and interconversion of nucleotide di- and triphosphates IEA Reactome





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