Gene description for Nrp2
Gene name neuropilin 2
Gene symbol Nrp2
Other names/aliases 1110048P06Rik
Np-2
Np2
Npn-2
Npn2
Species Mus musculus
 Database cross references - Nrp2
ExoCarta ExoCarta_18187
Entrez Gene 18187
UniProt O35375  
 Nrp2 identified in exosomes derived from the following tissue/cell type
Fibroblasts 23260141    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Mast cells 17486113    
 Gene ontology annotations for Nrp2
Molecular Function
    protein binding GO:0005515 IPI
    heparin binding GO:0008201 IEA
    metal ion binding GO:0046872 IEA
    semaphorin receptor activity GO:0017154 IDA
    vascular endothelial growth factor-activated receptor activity GO:0005021 IEA
Biological Process
    semaphorin-plexin signaling pathway GO:0071526 IDA
    vascular endothelial growth factor signaling pathway GO:0038084 IEA
    ganglion morphogenesis GO:0061552 IMP
    axon guidance GO:0007411 IMP
    axon extension involved in axon guidance GO:0048846 IMP
    sensory neuron axon guidance GO:0097374 IMP
    nervous system development GO:0007399 IEA
    gonadotrophin-releasing hormone neuronal migration to the hypothalamus GO:0021828 IMP
    multicellular organismal development GO:0007275 IEA
    ventral trunk neural crest cell migration GO:0036486 IMP
    nerve development GO:0021675 IMP
    neural crest cell migration involved in autonomic nervous system development GO:1901166 IMP
    sympathetic neuron projection guidance GO:0097491 IMP
    facial nerve structural organization GO:0021612 IMP
    vestibulocochlear nerve structural organization GO:0021649 IMP
    sympathetic ganglion development GO:0061549 IMP
    negative chemotaxis GO:0050919 IMP
    trigeminal ganglion development GO:0061551 IMP
    cell differentiation GO:0030154 IEA
    facioacoustic ganglion development GO:1903375 IMP
    sympathetic neuron projection extension GO:0097490 IMP
    heart development GO:0007507 IGI
    angiogenesis GO:0001525 IEA
    neuron migration GO:0001764 IMP
    neural crest cell migration GO:0001755 IMP
    semaphorin-plexin signaling pathway involved in neuron projection guidance GO:1902285 IMP
Subcellular Localization
    axon GO:0030424 IDA
    membrane GO:0016020 IEA
    plasma membrane GO:0005886 IC
    integral component of membrane GO:0016021 IEA
 Experiment description of studies that identified Nrp2 in exosomes
1
Experiment ID 210
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD81|FLOT1
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 214
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 215
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 216
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Nrp2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Plxna1  
Affinity Capture-Western Mus musculus
View the network image/svg+xml
 Pathways in which Nrp2 is involved
PathwayEvidenceSource
Neurophilin interactions with VEGF and VEGFR IEA Reactome
NrCAM interactions IEA Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here