Gene description for Nsdhl
Gene name NAD(P) dependent steroid dehydrogenase-like
Gene symbol Nsdhl
Other names/aliases AI747449
Bpa
H105E3
Str
XAP104
Species Mus musculus
 Database cross references - Nsdhl
ExoCarta ExoCarta_18194
Vesiclepedia VP_18194
Entrez Gene 18194
UniProt Q9R1J0  
 Nsdhl identified in sEVs derived from the following tissue/cell type
Neural stem cells 25242146    
 Gene ontology annotations for Nsdhl
Molecular Function
    C-3 sterol dehydrogenase (C-4 sterol decarboxylase) activity GO:0000252 IEA
    oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor GO:0016616 IBA
    3-beta-hydroxysteroid dehydrogenase/C4-decarboxylase activity GO:0102175 IEA
Biological Process
    hair follicle development GO:0001942 IMP
    cholesterol biosynthetic process GO:0006695 IEA
    smoothened signaling pathway GO:0007224 IGI
    cholesterol metabolic process GO:0008203 IBA
    cholesterol metabolic process GO:0008203 IMP
    labyrinthine layer blood vessel development GO:0060716 IMP
Subcellular Localization
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum GO:0005783 ISO
    endoplasmic reticulum membrane GO:0005789 IEA
    lipid droplet GO:0005811 IDA
    lipid droplet GO:0005811 ISO
 Experiment description of studies that identified Nsdhl in sEVs
1
Experiment ID 264
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th2 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Nsdhl
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Mcm7 17220
Co-fractionation Mus musculus
2 Usp7  
Affinity Capture-MS Mus musculus
3 Tmem173  
Proximity Label-MS Mus musculus
4 Lbr  
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Nsdhl is involved
PathwayEvidenceSource
Cholesterol biosynthesis IEA Reactome
Metabolism IEA Reactome
Metabolism of lipids IEA Reactome
Metabolism of steroids IEA Reactome





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