Gene description for Pdpk1
Gene name 3-phosphoinositide dependent protein kinase 1
Gene symbol Pdpk1
Other names/aliases Pdk1
Species Mus musculus
 Database cross references - Pdpk1
ExoCarta ExoCarta_18607
Vesiclepedia VP_18607
Entrez Gene 18607
UniProt Q9Z2A0  
 Pdpk1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Pdpk1
Molecular Function
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 ISO
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IDA
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IMP
    3-phosphoinositide-dependent protein kinase activity GO:0004676 ISO
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    insulin receptor binding GO:0005158 ISO
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    phospholipase activator activity GO:0016004 IEA
    phospholipase activator activity GO:0016004 ISO
    protein kinase binding GO:0019901 ISO
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    phospholipase binding GO:0043274 IEA
    phospholipase binding GO:0043274 ISO
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    type B pancreatic cell development GO:0003323 IMP
    chromatin remodeling GO:0006338 IEA
    hyperosmotic response GO:0006972 IDA
    signal transduction GO:0007165 TAS
    epidermal growth factor receptor signaling pathway GO:0007173 IEA
    insulin receptor signaling pathway GO:0008286 IDA
    insulin receptor signaling pathway GO:0008286 IMP
    regulation of endothelial cell migration GO:0010594 IMP
    negative regulation of cardiac muscle cell apoptotic process GO:0010667 IMP
    cell migration GO:0016477 IEA
    calcium-mediated signaling GO:0019722 IEA
    negative regulation of transforming growth factor beta receptor signaling pathway GO:0030512 IEA
    negative regulation of toll-like receptor signaling pathway GO:0034122 IMP
    intracellular signal transduction GO:0035556 IBA
    regulation of canonical NF-kappaB signal transduction GO:0043122 IEA
    regulation of mast cell degranulation GO:0043304 IMP
    negative regulation of neuron apoptotic process GO:0043524 ISO
    positive regulation of blood vessel endothelial cell migration GO:0043536 IEA
    insulin-like growth factor receptor signaling pathway GO:0048009 IDA
    insulin-like growth factor receptor signaling pathway GO:0048009 IMP
    focal adhesion assembly GO:0048041 ISO
    positive regulation of release of sequestered calcium ion into cytosol GO:0051281 IEA
    cellular response to epidermal growth factor stimulus GO:0071364 IEA
    extrinsic apoptotic signaling pathway GO:0097191 IEA
    positive regulation of protein localization to plasma membrane GO:1903078 IEA
    positive regulation of sprouting angiogenesis GO:1903672 IEA
    positive regulation of vascular endothelial cell proliferation GO:1905564 IEA
    negative regulation of endothelial cell apoptotic process GO:2000352 IEA
Subcellular Localization
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    focal adhesion GO:0005925 IEA
    postsynaptic density GO:0014069 IDA
    cytoplasmic vesicle GO:0031410 IDA
    cell projection GO:0042995 IEA
    cell projection GO:0042995 ISO
    perikaryon GO:0043204 ISO
 Experiment description of studies that identified Pdpk1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Pdpk1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Runx2  
Affinity Capture-MS Mus musculus
2 PRKACB  
Affinity Capture-MS Bos taurus
3 Ubc  
Reconstituted Complex Mus musculus
View the network image/svg+xml
 Pathways in which Pdpk1 is involved
PathwayEvidenceSource
Activation of AKT2 IEA Reactome
Adaptive Immune System IEA Reactome
C-type lectin receptors (CLRs) IEA Reactome
CD28 co-stimulation IEA Reactome
CD28 dependent PI3K/Akt signaling IEA Reactome
Cellular responses to mechanical stimuli IEA Reactome
Cellular responses to stimuli IEA Reactome
CLEC7A (Dectin-1) signaling IEA Reactome
Costimulation by the CD28 family IEA Reactome
Downstream TCR signaling IEA Reactome
ESR-mediated signaling IEA Reactome
Estrogen-stimulated signaling through PRKCZ IEA Reactome
Extra-nuclear estrogen signaling IEA Reactome
Fc epsilon receptor (FCERI) signaling IEA Reactome
FCERI mediated NF-kB activation IEA Reactome
G beta:gamma signalling through PI3Kgamma IEA Reactome
G-protein beta:gamma signalling IEA Reactome
Gene expression (Transcription) IEA Reactome
Generic Transcription Pathway IEA Reactome
GPCR downstream signalling IEA Reactome
GPVI-mediated activation cascade IEA Reactome
Hemostasis IEA Reactome
High laminar flow shear stress activates signaling by PIEZO1 and PECAM1:CDH5:KDR in endothelial cells IEA Reactome
IGF1R signaling cascade IEA Reactome
Immune System IEA Reactome
Innate Immune System IEA Reactome
Insulin receptor signalling cascade IEA Reactome
Integrin signaling IEA Reactome
Intracellular signaling by second messengers IEA Reactome
IRS-mediated signalling IEA Reactome
IRS-related events triggered by IGF1R IEA Reactome
PI3K Cascade IEA Reactome
PIP3 activates AKT signaling IEA Reactome
Platelet activation, signaling and aggregation IEA Reactome
Platelet Aggregation (Plug Formation) IEA Reactome
Regulation of TP53 Activity IEA Reactome
Regulation of TP53 Degradation IEA Reactome
Regulation of TP53 Expression and Degradation IEA Reactome
Response of endothelial cells to shear stress IEA Reactome
RHO GTPase Effectors IEA Reactome
RHO GTPases activate PKNs IEA Reactome
RNA Polymerase II Transcription IEA Reactome
Role of LAT2/NTAL/LAB on calcium mobilization IEA Reactome
Signal Transduction IEA Reactome
Signaling by GPCR IEA Reactome
Signaling by Insulin receptor IEA Reactome
Signaling by Nuclear Receptors IEA Reactome
Signaling by Receptor Tyrosine Kinases IEA Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome
Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R) IEA Reactome
Signaling by VEGF IEA Reactome
TCR signaling IEA Reactome
Transcriptional Regulation by TP53 IEA Reactome
VEGFA-VEGFR2 Pathway IEA Reactome
VEGFR2 mediated cell proliferation IEA Reactome
VEGFR2 mediated vascular permeability IEA Reactome





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