Gene description for Phf2
Gene name PHD finger protein 2
Gene symbol Phf2
Other names/aliases GRC5
Species Mus musculus
 Database cross references - Phf2
ExoCarta ExoCarta_18676
Vesiclepedia VP_18676
Entrez Gene 18676
UniProt Q9WTU0  
 Phf2 identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Phf2
Molecular Function
    transcription coregulator activity GO:0003712 IBA
    transcription coactivator activity GO:0003713 ISO
    transcription coactivator activity GO:0003713 ISS
    iron ion binding GO:0005506 ISO
    iron ion binding GO:0005506 ISS
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 ISO
    zinc ion binding GO:0008270 ISS
    histone demethylase activity GO:0032452 IBA
    histone H3K9 demethylase activity GO:0032454 ISO
    histone H3K9 demethylase activity GO:0032454 ISS
    histone H3K9 demethylase activity GO:0032454 TAS
    methylated histone binding GO:0035064 ISO
    methylated histone binding GO:0035064 ISS
    histone H4K20 demethylase activity GO:0035575 IMP
Biological Process
    chromatin remodeling GO:0006338 IBA
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    protein demethylation GO:0006482 ISO
    protein demethylation GO:0006482 ISS
    transcription initiation-coupled chromatin remodeling GO:0045815 IMP
    positive regulation of DNA-templated transcription GO:0045893 IEA
    negative regulation of rDNA heterochromatin formation GO:0061188 ISO
    negative regulation of rDNA heterochromatin formation GO:0061188 ISS
Subcellular Localization
    kinetochore GO:0000776 ISO
    kinetochore GO:0000776 ISS
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    nucleolus GO:0005730 ISO
    nucleolus GO:0005730 ISS
 Experiment description of studies that identified Phf2 in sEVs
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Phf2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Maml1  
Two-hybrid Mus musculus
2 JADE1  
Affinity Capture-MS Homo sapiens
3 Foxp1  
Two-hybrid Mus musculus
4 Tox3  
Two-hybrid Mus musculus
5 Twist1  
Proximity Label-MS Mus musculus
6 Tcf3  
Two-hybrid Mus musculus
View the network image/svg+xml
 Pathways in which Phf2 is involved
PathwayEvidenceSource
Chromatin modifying enzymes IEA Reactome
Chromatin organization IEA Reactome
HDMs demethylate histones IEA Reactome





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