Gene description for Pitx2
Gene name paired-like homeodomain transcription factor 2
Gene symbol Pitx2
Other names/aliases 9430085M16Rik
Brx1
Brx1b
Munc30
Otlx2
Ptx2
Rieg
Species Mus musculus
 Database cross references - Pitx2
ExoCarta ExoCarta_18741
Entrez Gene 18741
UniProt P97474  
 Pitx2 identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Pitx2
Molecular Function
    chromatin DNA binding GO:0031490 IDA
    sequence-specific DNA binding GO:0043565 IDA
    RNA polymerase II activating transcription factor binding GO:0001102 ISO
    phosphoprotein binding GO:0051219 ISO
    chromatin binding GO:0003682 IDA
    RNA polymerase II transcription coactivator activity GO:0001105 ISO
    RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription GO:0001077 ISO
    RNA polymerase II core promoter proximal region sequence-specific DNA binding GO:0000978 ISO
    protein binding GO:0005515 IPI
    RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription GO:0001191 ISO
    RNA polymerase II transcription factor binding GO:0001085 ISO
    identical protein binding GO:0042802 ISO
    sequence-specific DNA binding transcription factor activity GO:0003700 ISO
    RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription GO:0001078 ISO
    transcription factor binding GO:0008134 ISO
    sequence-specific DNA binding RNA polymerase II transcription factor activity GO:0000981 IDA
    transcription regulatory region sequence-specific DNA binding GO:0000976 ISO
    protein homodimerization activity GO:0042803 ISO
    DNA binding GO:0003677 IDA
Biological Process
    cardiac muscle cell differentiation GO:0055007 IMP
    atrial cardiac muscle tissue morphogenesis GO:0055009 IMP
    endodermal digestive tract morphogenesis GO:0061031 IMP
    positive regulation of myoblast proliferation GO:2000288 IDA
    spleen development GO:0048536 IMP
    regulation of cell proliferation GO:0042127 IMP
    left/right axis specification GO:0070986 IMP
    subthalamic nucleus development GO:0021763 IMP
    extraocular skeletal muscle development GO:0002074 IMP
    in utero embryonic development GO:0001701 IMP
    organ morphogenesis GO:0009887 IMP
    skeletal muscle tissue development GO:0007519 IMP
    embryonic hindlimb morphogenesis GO:0035116 IGI
    left lung morphogenesis GO:0060460 IMP
    cardiac muscle tissue development GO:0048738 IMP
    anatomical structure morphogenesis GO:0009653 IMP
    neuron migration GO:0001764 IMP
    vascular smooth muscle cell differentiation GO:0035886 IMP
    determination of left/right symmetry GO:0007368 IMP
    multicellular organismal development GO:0007275 IEA
    odontogenesis GO:0042476 ISO
    ventricular septum morphogenesis GO:0060412 IMP
    pituitary gland development GO:0021983 IMP
    vasculogenesis GO:0001570 IMP
    hypothalamus cell migration GO:0021855 IMP
    regulation of transcription, DNA-templated GO:0006355 ISO
    pulmonary vein morphogenesis GO:0060577 IMP
    embryonic camera-type eye development GO:0031076 IMP
    atrioventricular valve development GO:0003171 IMP
    regulation of cell migration GO:0030334 IMP
    ventricular cardiac muscle cell development GO:0055015 IMP
    superior vena cava morphogenesis GO:0060578 IMP
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 ISO
    regulation of transcription from RNA polymerase II promoter GO:0006357 ISO
    positive regulation of transcription, DNA-templated GO:0045893 IMP
    Wnt signaling pathway GO:0016055 IDA
    positive regulation of DNA binding GO:0043388 IDA
    cardiac neural crest cell migration involved in outflow tract morphogenesis GO:0003253 IMP
    patterning of blood vessels GO:0001569 IMP
    lung development GO:0030324 IMP
    digestive system development GO:0055123 IMP
    deltoid tuberosity development GO:0035993 ISO
    cell proliferation involved in outflow tract morphogenesis GO:0061325 IMP
    myoblast fusion GO:0007520 IDA
    iris morphogenesis GO:0061072 ISO
    transcription from RNA polymerase II promoter GO:0006366 IDA
    embryonic digestive tract morphogenesis GO:0048557 IMP
    heart development GO:0007507 IMP
    camera-type eye development GO:0043010 ISO
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 ISO
    pulmonary myocardium development GO:0003350 IMP
Subcellular Localization
    cytoplasm GO:0005737 IDA
    nucleoplasm GO:0005654 ISO
    transcription factor complex GO:0005667 ISO
    nucleus GO:0005634 ISO
 Experiment description of studies that identified Pitx2 in exosomes
1
Experiment ID 15
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Pitx2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Pitx2 is involved
No pathways found





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