Gene description for Prkch
Gene name protein kinase C, eta
Gene symbol Prkch
Other names/aliases Pkch
Species Mus musculus
 Database cross references - Prkch
ExoCarta ExoCarta_18755
Vesiclepedia VP_18755
Entrez Gene 18755
UniProt P23298  
 Prkch identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Prkch
Molecular Function
    protein kinase activity GO:0004672 ISO
    protein serine/threonine kinase activity GO:0004674 IBA
    calcium,diacylglycerol-dependent serine/threonine kinase activity GO:0004698 IEA
    diacylglycerol-dependent, calcium-independent serine/threonine kinase activity GO:0004699 IEA
    diacylglycerol-dependent, calcium-independent serine/threonine kinase activity GO:0004699 ISO
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    enzyme binding GO:0019899 ISO
    protein serine/threonine kinase inhibitor activity GO:0030291 ISO
    small GTPase binding GO:0031267 IPI
    translation regulator activity GO:0045182 ISO
    metal ion binding GO:0046872 IEA
    protein serine kinase activity GO:0106310 IEA
    protein serine/threonine kinase binding GO:0120283 ISO
Biological Process
    protein phosphorylation GO:0006468 ISO
    protein phosphorylation GO:0006468 ISS
    positive regulation of macrophage derived foam cell differentiation GO:0010744 IMP
    negative regulation of translation GO:0017148 ISO
    cell differentiation GO:0030154 IEA
    positive regulation of translation in response to stress GO:0032056 ISO
    cellular response to amino acid starvation GO:0034198 ISO
    negative regulation of glial cell apoptotic process GO:0034351 ISO
    negative regulation of glial cell apoptotic process GO:0034351 ISS
    intracellular signal transduction GO:0035556 IBA
    positive regulation of keratinocyte differentiation GO:0045618 IMP
    positive regulation of B cell receptor signaling pathway GO:0050861 ISO
    positive regulation of B cell receptor signaling pathway GO:0050861 ISS
    positive regulation of NF-kappaB transcription factor activity GO:0051092 ISO
    positive regulation of NF-kappaB transcription factor activity GO:0051092 ISS
    positive regulation of glial cell proliferation GO:0060252 ISO
    positive regulation of glial cell proliferation GO:0060252 ISS
    protein kinase C signaling GO:0070528 IEA
    protein kinase C signaling GO:0070528 ISO
    negative regulation of protein serine/threonine kinase activity GO:0071901 ISO
    regulation of bicellular tight junction assembly GO:2000810 ISO
    regulation of bicellular tight junction assembly GO:2000810 ISS
Subcellular Localization
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    cell-cell junction GO:0005911 IDA
 Experiment description of studies that identified Prkch in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Prkch
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MTNR1A  
Reconstituted Complex Homo sapiens
2 Tmem173  
Proximity Label-MS Mus musculus
View the network image/svg+xml



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