Gene description for Pklr
Gene name pyruvate kinase liver and red blood cell
Gene symbol Pklr
Other names/aliases Pk-1
Pk1
R-PK
Species Mus musculus
 Database cross references - Pklr
ExoCarta ExoCarta_18770
Vesiclepedia VP_18770
Entrez Gene 18770
 Pklr identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Pklr
Molecular Function
    magnesium ion binding GO:0000287 IEA
    pyruvate kinase activity GO:0004743 IBA
    pyruvate kinase activity GO:0004743 IDA
    pyruvate kinase activity GO:0004743 ISO
    ATP binding GO:0005524 IEA
    kinase activity GO:0016301 IEA
    potassium ion binding GO:0030955 IEA
    monosaccharide binding GO:0048029 ISO
Biological Process
    glycolytic process GO:0006096 IBA
    glycolytic process GO:0006096 IDA
    glycolytic process GO:0006096 IEA
    glycolytic process GO:0006096 ISO
    glycolytic process GO:0006096 NAS
    cellular response to insulin stimulus GO:0032869 IBA
    cellular response to insulin stimulus GO:0032869 ISO
    response to ATP GO:0033198 ISO
    pyruvate biosynthetic process GO:0042866 ISO
    response to cAMP GO:0051591 ISO
    response to other organism GO:0051707 IMP
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    pyruvate kinase complex GO:1902912 ISO
 Experiment description of studies that identified Pklr in sEVs
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Pklr
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Smurf2  
Affinity Capture-Western Mus musculus
2 Dvl2  
Affinity Capture-Western Mus musculus
3 Pard6a  
Affinity Capture-Western Mus musculus
View the network image/svg+xml
 Pathways in which Pklr is involved
No pathways found





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