Gene description for Pklr
Gene name pyruvate kinase liver and red blood cell
Gene symbol Pklr
Other names/aliases Pk-1
Pk1
R-PK
Species Mus musculus
 Database cross references - Pklr
ExoCarta ExoCarta_18770
Entrez Gene 18770
 Pklr identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Pklr
Molecular Function
    transferase activity GO:0016740 IEA
    catalytic activity GO:0003824 IEA
    magnesium ion binding GO:0000287 IEA
    metal ion binding GO:0046872 IEA
    pyruvate kinase activity GO:0004743 ISO
    ATP binding GO:0005524 IEA
    kinase activity GO:0016301 IEA
    potassium ion binding GO:0030955 IEA
    nucleotide binding GO:0000166 IEA
Biological Process
    carbohydrate metabolic process GO:0005975 ISO
    response to other organism GO:0051707 IMP
    phosphorylation GO:0016310 IDA
    response to heat GO:0009408 ISO
    response to cAMP GO:0051591 ISO
    response to ATP GO:0033198 ISO
    response to lithium ion GO:0010226 ISO
    cellular response to insulin stimulus GO:0032869 ISO
    glycolytic process GO:0006096 ISO
    metabolic process GO:0008152 IEA
    pyruvate biosynthetic process GO:0042866 ISO
    ATP biosynthetic process GO:0006754 ISO
Subcellular Localization
    cytosol GO:0005829 ISO
    extracellular exosome GO:0070062 ISO
 Experiment description of studies that identified Pklr in exosomes
1
Experiment ID 15
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Pklr
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Pklr is involved
No pathways found





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