Gene description for Serpine1
Gene name serine (or cysteine) peptidase inhibitor, clade E, member 1
Gene symbol Serpine1
Other names/aliases PAI-1
PAI1
Planh1
Species Mus musculus
 Database cross references - Serpine1
ExoCarta ExoCarta_18787
Vesiclepedia VP_18787
Entrez Gene 18787
 Serpine1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mov neuroglial cells 15210972    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Serpine1
Molecular Function
    protease binding GO:0002020 ISO
    serine-type endopeptidase inhibitor activity GO:0004867 IBA
    serine-type endopeptidase inhibitor activity GO:0004867 IDA
    serine-type endopeptidase inhibitor activity GO:0004867 ISO
    signaling receptor binding GO:0005102 ISO
    protein binding GO:0005515 IPI
Biological Process
    angiogenesis GO:0001525 IMP
    placenta development GO:0001890 IMP
    female gonad development GO:0008585 ISO
    regulation of signaling receptor activity GO:0010469 ISO
    positive regulation of gene expression GO:0010628 ISO
    negative regulation of gene expression GO:0010629 ISO
    negative regulation of plasminogen activation GO:0010757 IBA
    negative regulation of plasminogen activation GO:0010757 IMP
    negative regulation of plasminogen activation GO:0010757 ISO
    negative regulation of plasminogen activation GO:0010757 NAS
    negative regulation of endopeptidase activity GO:0010951 IBA
    negative regulation of endopeptidase activity GO:0010951 ISO
    negative regulation of smooth muscle cell migration GO:0014912 ISO
    positive regulation of blood coagulation GO:0030194 ISO
    negative regulation of cell migration GO:0030336 ISO
    positive regulation of interleukin-8 production GO:0032757 ISO
    positive regulation of collagen biosynthetic process GO:0032967 ISO
    negative regulation of cell adhesion mediated by integrin GO:0033629 ISO
    positive regulation of leukotriene production involved in inflammatory response GO:0035491 ISO
    regulation of cell population proliferation GO:0042127 IGI
    regulation of angiogenesis GO:0045765 IDA
    positive regulation of angiogenesis GO:0045766 IMP
    positive regulation of angiogenesis GO:0045766 IMP
    positive regulation of angiogenesis GO:0045766 ISO
    positive regulation of fibroblast proliferation GO:0048146 ISO
    positive regulation of receptor-mediated endocytosis GO:0048260 ISO
    positive regulation of inflammatory response GO:0050729 ISO
    positive regulation of coagulation GO:0050820 ISO
    defense response to Gram-negative bacterium GO:0050829 IMP
    defense response to Gram-negative bacterium GO:0050829 ISO
    positive regulation of keratinocyte migration GO:0051549 ISO
    negative regulation of fibrinolysis GO:0051918 ISO
    negative regulation of fibrinolysis GO:0051918 NAS
    negative regulation of vascular wound healing GO:0061044 IBA
    negative regulation of vascular wound healing GO:0061044 IMP
    negative regulation of vascular wound healing GO:0061044 ISO
    cellular response to lipopolysaccharide GO:0071222 ISO
    cellular response to interleukin-1 GO:0071347 ISO
    cellular response to xenobiotic stimulus GO:0071466 ISO
    cellular response to transforming growth factor beta stimulus GO:0071560 IDA
    positive regulation of monocyte chemotaxis GO:0090026 ISO
    positive regulation of calcium ion import GO:0090280 ISO
    replicative senescence GO:0090399 ISO
    dentinogenesis GO:0097187 ISO
    positive regulation of odontoblast differentiation GO:1901331 ISO
    negative regulation of extrinsic apoptotic signaling pathway via death domain receptors GO:1902042 ISO
    positive regulation of epithelium regeneration GO:1905043 ISO
    negative regulation of smooth muscle cell-matrix adhesion GO:2000098 ISO
    negative regulation of endothelial cell apoptotic process GO:2000352 ISO
Subcellular Localization
    extracellular region GO:0005576 ISO
    extracellular space GO:0005615 HDA
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 ISO
    chromaffin granule GO:0042583 ISO
    collagen-containing extracellular matrix GO:0062023 ISO
    collagen-containing extracellular matrix GO:0062023 NAS
    extracellular exosome GO:0070062 ISO
    serine protease inhibitor complex GO:0097180 ISO
    peptidase inhibitor complex GO:1904090 ISO
 Experiment description of studies that identified Serpine1 in sEVs
1
Experiment ID 907
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 39
MISEV standards
IEM
Biophysical techniques
TSG101|HSC70|FLOT1
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors "Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G"
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
3
Experiment ID 908
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Serpine1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tmprss11e  
Reconstituted Complex Mus musculus
2 Dyrk1b  
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Serpine1 is involved
No pathways found





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