Gene description for Plcb1
Gene name phospholipase C, beta 1
Gene symbol Plcb1
Other names/aliases 3110043I21Rik
AI132408
Plcb
mKIAA0581
Species Mus musculus
 Database cross references - Plcb1
ExoCarta ExoCarta_18795
Vesiclepedia VP_18795
Entrez Gene 18795
UniProt Q9Z1B3  
 Plcb1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Plcb1
Molecular Function
    phosphatidylinositol phospholipase C activity GO:0004435 IBA
    phosphatidylinositol phospholipase C activity GO:0004435 IDA
    phosphatidylinositol phospholipase C activity GO:0004435 ISO
    phosphatidylinositol phospholipase C activity GO:0004435 ISS
    GTPase activator activity GO:0005096 IEA
    GTPase activator activity GO:0005096 ISO
    calcium ion binding GO:0005509 IEA
    calcium ion binding GO:0005509 ISO
    protein binding GO:0005515 IPI
    calmodulin binding GO:0005516 IBA
    calmodulin binding GO:0005516 ISO
    calmodulin binding GO:0005516 ISS
    lamin binding GO:0005521 IPI
    phosphatidylinositol-4,5-bisphosphate binding GO:0005546 ISO
    phosphatidylinositol-4,5-bisphosphate binding GO:0005546 ISS
    enzyme binding GO:0019899 ISO
    enzyme binding GO:0019899 ISS
    identical protein binding GO:0042802 ISO
    identical protein binding GO:0042802 ISS
Biological Process
    G2/M transition of mitotic cell cycle GO:0000086 IDA
    G2/M transition of mitotic cell cycle GO:0000086 NAS
    oocyte maturation GO:0001556 NAS
    mRNA processing GO:0006397 TAS
    cell adhesion GO:0007155 IC
    signal transduction GO:0007165 TAS
    G protein-coupled receptor signaling pathway GO:0007186 IBA
    phospholipase C-activating G protein-coupled receptor signaling pathway GO:0007200 IMP
    phospholipase C-activating G protein-coupled acetylcholine receptor signaling pathway GO:0007207 IGI
    phospholipase C-activating G protein-coupled acetylcholine receptor signaling pathway GO:0007207 IMP
    G protein-coupled acetylcholine receptor signaling pathway GO:0007213 IMP
    glutamate receptor signaling pathway GO:0007215 IMP
    brain development GO:0007420 NAS
    learning GO:0007612 IEA
    memory GO:0007613 IBA
    memory GO:0007613 IMP
    regulation of G protein-coupled receptor signaling pathway GO:0008277 IMP
    insulin receptor signaling pathway GO:0008286 TAS
    cerebral cortex development GO:0021987 IMP
    erythrocyte differentiation GO:0030218 TAS
    macrophage differentiation GO:0030225 NAS
    phosphatidylinositol catabolic process GO:0031161 IEA
    phosphatidylinositol catabolic process GO:0031161 ISO
    positive regulation of insulin secretion GO:0032024 IGI
    positive regulation of insulin secretion GO:0032024 IMP
    positive regulation of sodium:proton antiporter activity GO:0032417 NAS
    positive regulation of interleukin-12 production GO:0032735 IMP
    inositol trisphosphate metabolic process GO:0032957 IEA
    inositol trisphosphate metabolic process GO:0032957 ISO
    response to monosaccharide GO:0034284 ISO
    intracellular signal transduction GO:0035556 IEA
    interleukin-12-mediated signaling pathway GO:0035722 ISO
    interleukin-12-mediated signaling pathway GO:0035722 ISS
    interleukin-15-mediated signaling pathway GO:0035723 ISO
    interleukin-15-mediated signaling pathway GO:0035723 ISS
    positive regulation of embryonic development GO:0040019 IMP
    response to peptide hormone GO:0043434 ISO
    fat cell differentiation GO:0045444 IDA
    positive regulation of myoblast differentiation GO:0045663 IDA
    negative regulation of DNA-templated transcription GO:0045892 IDA
    positive regulation of DNA-templated transcription GO:0045893 IDA
    positive regulation of JNK cascade GO:0046330 ISO
    positive regulation of JNK cascade GO:0046330 ISS
    phosphatidylinositol metabolic process GO:0046488 IBA
    phosphatidylinositol metabolic process GO:0046488 ISO
    phosphatidylinositol metabolic process GO:0046488 ISS
    insulin-like growth factor receptor signaling pathway GO:0048009 IDA
    phosphatidylinositol-mediated signaling GO:0048015 IBA
    positive regulation of developmental growth GO:0048639 IMP
    release of sequestered calcium ion into cytosol GO:0051209 IBA
    activation of meiosis involved in egg activation GO:0060466 IDA
    interleukin-1-mediated signaling pathway GO:0070498 IEA
    interleukin-1-mediated signaling pathway GO:0070498 ISO
    regulation of fertilization GO:0080154 IMP
    ion channel modulating, G protein-coupled receptor signaling pathway GO:0099105 IMP
    postsynaptic modulation of chemical synaptic transmission GO:0099170 EXP
    postsynaptic modulation of chemical synaptic transmission GO:0099170 IDA
    postsynaptic modulation of chemical synaptic transmission GO:0099170 IMP
    regulation of retrograde trans-synaptic signaling by endocanabinoid GO:0099178 IDA
    regulation of retrograde trans-synaptic signaling by endocanabinoid GO:0099178 IMP
    positive regulation of G1/S transition of mitotic cell cycle GO:1900087 IDA
    cellular response to fluoride GO:1902618 IEA
    cellular response to fluoride GO:1902618 ISO
    regulation of establishment of endothelial barrier GO:1903140 IMP
    cellular response to vasopressin GO:1904117 IEA
    cellular response to vasopressin GO:1904117 ISO
    cellular response to ionomycin GO:1904637 IEA
    cellular response to ionomycin GO:1904637 ISO
    cellular response to glyceraldehyde GO:1905631 IEA
    cellular response to glyceraldehyde GO:1905631 ISO
    ligand-gated ion channel signaling pathway GO:1990806 IMP
    positive regulation of acrosome reaction GO:2000344 IMP
    negative regulation of monocyte extravasation GO:2000438 IDA
    positive regulation of CD24 production GO:2000560 IDA
Subcellular Localization
    chromatin GO:0000785 IDA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 NAS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 TAS
    nuclear speck GO:0016607 IBA
    nuclear speck GO:0016607 IDA
    nuclear speck GO:0016607 ISO
    nuclear membrane GO:0031965 IEA
    protein-containing complex GO:0032991 IDA
    myelin sheath GO:0043209 HDA
    glutamatergic synapse GO:0098978 EXP
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
    GABA-ergic synapse GO:0098982 IDA
    GABA-ergic synapse GO:0098982 IMP
    postsynaptic cytosol GO:0099524 IDA
    postsynaptic cytosol GO:0099524 IEP
 Experiment description of studies that identified Plcb1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Plcb1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Shank3  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
2 Sstr3  
Proximity Label-MS Mus musculus
View the network image/svg+xml



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