Gene description for Plod1
Gene name procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1
Gene symbol Plod1
Other names/aliases 2410042F05Rik
AI854890
AV007929
Lh1
Plod
Species Mus musculus
 Database cross references - Plod1
ExoCarta ExoCarta_18822
Vesiclepedia VP_18822
Entrez Gene 18822
UniProt Q9R0E2  
 Plod1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Plod1
Molecular Function
    iron ion binding GO:0005506 IEA
    protein binding GO:0005515 IPI
    ferrous iron binding GO:0008198 ISO
    procollagen-lysine 5-dioxygenase activity GO:0008475 IBA
    procollagen-lysine 5-dioxygenase activity GO:0008475 ISO
    procollagen-lysine 5-dioxygenase activity GO:0008475 ISS
    L-ascorbic acid binding GO:0031418 IEA
    L-ascorbic acid binding GO:0031418 ISO
    peptide binding GO:0042277 ISO
Biological Process
    response to hypoxia GO:0001666 IEA
    epidermis development GO:0008544 IEA
    epidermis development GO:0008544 ISO
    peptidyl-lysine hydroxylation GO:0017185 ISO
    peptidyl-lysine hydroxylation GO:0017185 ISS
    collagen fibril organization GO:0030199 IBA
Subcellular Localization
    extracellular space GO:0005615 IBA
    endoplasmic reticulum GO:0005783 IBA
    Golgi apparatus GO:0005794 IBA
    rough endoplasmic reticulum membrane GO:0030867 IEA
    collagen-containing extracellular matrix GO:0062023 HDA
    collagen-containing extracellular matrix GO:0062023 IBA
    catalytic complex GO:1902494 IMP
 Experiment description of studies that identified Plod1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Plod1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Cand2  
Co-fractionation Mus musculus
2 Eed  
Affinity Capture-MS Mus musculus
3 Plod2  
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Plod1 is involved
PathwayEvidenceSource
Collagen biosynthesis and modifying enzymes IEA Reactome
Collagen formation IEA Reactome
Extracellular matrix organization IEA Reactome





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