Gene description for Pou4f1
Gene name POU domain, class 4, transcription factor 1
Gene symbol Pou4f1
Other names/aliases Brn-3
Brn-3.0
Brn3
Brn3.0
Brn3a
E130119J07Rik
Species Mus musculus
 Database cross references - Pou4f1
ExoCarta ExoCarta_18996
Vesiclepedia VP_18996
Entrez Gene 18996
UniProt P17208  
 Pou4f1 identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Pou4f1
Molecular Function
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IBA
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IDA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IDA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 ISO
    DNA-binding transcription repressor activity, RNA polymerase II-specific GO:0001227 IGI
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 IDA
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 IGI
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 IMP
    DNA binding GO:0003677 IDA
    chromatin binding GO:0003682 IGI
    single-stranded DNA binding GO:0003697 IDA
    DNA-binding transcription factor activity GO:0003700 IMP
    protein binding GO:0005515 IPI
    sequence-specific DNA binding GO:0043565 IDA
    GTPase binding GO:0051020 IPI
    RNA polymerase II-specific DNA-binding transcription factor binding GO:0061629 IPI
    RNA polymerase II-specific DNA-binding transcription factor binding GO:0061629 ISO
    sequence-specific double-stranded DNA binding GO:1990837 ISO
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IDA
    negative regulation of transcription by RNA polymerase II GO:0000122 IGI
    negative regulation of transcription by RNA polymerase II GO:0000122 IMP
    negative regulation of transcription by RNA polymerase II GO:0000122 IMP
    neuron migration GO:0001764 IMP
    suckling behavior GO:0001967 IMP
    ventricular compact myocardium morphogenesis GO:0003223 IMP
    regulation of DNA-templated transcription GO:0006355 IMP
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    nervous system development GO:0007399 IDA
    mesoderm development GO:0007498 IMP
    heart development GO:0007507 IGI
    positive regulation of gene expression GO:0010628 IDA
    negative regulation of gene expression GO:0010629 IMP
    cell migration in hindbrain GO:0021535 IMP
    trigeminal nerve development GO:0021559 IMP
    central nervous system neuron differentiation GO:0021953 IMP
    habenula development GO:0021986 IMP
    neuron differentiation GO:0030182 IMP
    neuron projection development GO:0031175 IDA
    negative regulation of apoptotic process GO:0043066 IGI
    negative regulation of apoptotic process GO:0043066 IGI
    negative regulation of programmed cell death GO:0043069 IMP
    negative regulation of neuron apoptotic process GO:0043524 IMP
    negative regulation of neuron apoptotic process GO:0043524 ISO
    negative regulation of neuron apoptotic process GO:0043524 ISS
    positive regulation of neuron apoptotic process GO:0043525 IGI
    positive regulation of osteoclast differentiation GO:0045672 IMP
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IGI
    positive regulation of transcription by RNA polymerase II GO:0045944 IMP
    positive regulation of transcription by RNA polymerase II GO:0045944 IMP
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    neuron fate specification GO:0048665 IMP
    sensory system development GO:0048880 IMP
    peripheral nervous system neuron differentiation GO:0048934 IMP
    peripheral nervous system neuron development GO:0048935 IGI
    peripheral nervous system neuron development GO:0048935 IMP
    regulation of neurogenesis GO:0050767 IMP
    regulation of DNA-binding transcription factor activity GO:0051090 IDA
    proprioception involved in equilibrioception GO:0051355 IMP
    neuron apoptotic process GO:0051402 IGI
    regulation of cell cycle GO:0051726 IGI
    regulation of cell cycle GO:0051726 IGI
    regulation of cell cycle GO:0051726 IMP
    innervation GO:0060384 IMP
    cellular response to cytokine stimulus GO:0071345 IDA
    cellular response to estradiol stimulus GO:0071392 IDA
    intrinsic apoptotic signaling pathway by p53 class mediator GO:0072332 IMP
    positive regulation of transcription regulatory region DNA binding GO:2000679 IDA
Subcellular Localization
    chromatin GO:0000785 IDA
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
    neuron projection GO:0043005 IDA
    RNA polymerase II transcription regulator complex GO:0090575 IDA
 Experiment description of studies that identified Pou4f1 in sEVs
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Pou4f1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Esr1  
Two-hybrid Mus musculus
2 Hipk2  
Affinity Capture-Western Mus musculus
3 Usp7  
Affinity Capture-MS Mus musculus
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