Gene description for Prep
Gene name prolyl endopeptidase
Gene symbol Prep
Other names/aliases AI047692
AI450383
D10Wsu136e
PEP
Pop
Species Mus musculus
 Database cross references - Prep
ExoCarta ExoCarta_19072
Vesiclepedia VP_19072
Entrez Gene 19072
UniProt Q9QUR6  
 Prep identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Prep
Molecular Function
    endopeptidase activity GO:0004175 ISO
    metallocarboxypeptidase activity GO:0004181 IMP
    serine-type endopeptidase activity GO:0004252 IEA
    peptidase activity GO:0008233 ISS
    peptide binding GO:0042277 ISO
    oligopeptidase activity GO:0070012 IBA
Biological Process
    angiotensin maturation GO:0002003 IMP
    proteolysis GO:0006508 IEA
    protein metabolic process GO:0019538 ISS
    protein catabolic process GO:0030163 ISO
Subcellular Localization
    extracellular region GO:0005576 IDA
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
 Experiment description of studies that identified Prep in sEVs
1
Experiment ID 907
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Prep
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fancd2  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Prep is involved
No pathways found





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