Gene description for Nme1
Gene name NME/NM23 nucleoside diphosphate kinase 1
Gene symbol Nme1
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Nme1
ExoCarta ExoCarta_191575
Vesiclepedia VP_191575
Entrez Gene 191575
UniProt Q05982  
 Nme1 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Urine 20535238    
 Gene ontology annotations for Nme1
Molecular Function
    magnesium ion binding GO:0000287 ISO
    RNA polymerase II transcription regulatory region sequence-specific DNA binding GO:0000977 IDA
    single-stranded DNA binding GO:0003697 IDA
    DNA nuclease activity GO:0004536 ISO
    nucleoside diphosphate kinase activity GO:0004550 IBA
    nucleoside diphosphate kinase activity GO:0004550 IDA
    nucleoside diphosphate kinase activity GO:0004550 IEA
    nucleoside diphosphate kinase activity GO:0004550 ISO
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP binding GO:0005524 ISO
    GTP binding GO:0005525 ISO
    3'-5' exonuclease activity GO:0008408 ISO
    intermediate filament binding GO:0019215 IPI
    enzyme binding GO:0019899 IPI
    identical protein binding GO:0042802 ISO
    gamma-tubulin binding GO:0043015 IDA
    ribosomal small subunit binding GO:0043024 ISO
Biological Process
    negative regulation of myeloid leukocyte differentiation GO:0002762 IDA
    GTP biosynthetic process GO:0006183 IDA
    GTP biosynthetic process GO:0006183 IEA
    UTP biosynthetic process GO:0006228 IEA
    CTP biosynthetic process GO:0006241 IEA
    DNA metabolic process GO:0006259 IEA
    endocytosis GO:0006897 IEA
    nervous system development GO:0007399 IEA
    lactation GO:0007595 ISO
    response to xenobiotic stimulus GO:0009410 IEP
    negative regulation of gene expression GO:0010629 IDA
    positive regulation of neuron projection development GO:0010976 IMP
    response to amine GO:0014075 IEP
    hippocampus development GO:0021766 IEP
    cell differentiation GO:0030154 IEA
    mammary gland development GO:0030879 ISO
    response to testosterone GO:0033574 IEP
    positive regulation of epithelial cell proliferation GO:0050679 ISO
    response to cAMP GO:0051591 IEP
    cellular response to glucose stimulus GO:0071333 IEP
    cellular response to fatty acid GO:0071398 IEP
    cellular response to xenobiotic stimulus GO:0071466 IEP
Subcellular Localization
    nucleus GO:0005634 IEA
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 IEA
    mitochondrial outer membrane GO:0005741 IDA
    early endosome GO:0005769 ISO
    centrosome GO:0005813 IDA
    cytosol GO:0005829 ISO
    intermediate filament GO:0005882 IDA
    ruffle membrane GO:0032587 ISO
    perinuclear region of cytoplasm GO:0048471 IDA
 Experiment description of studies that identified Nme1 in exosomes
1
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 192
MISEV standards
CEM
Biophysical techniques
TSG101|HSP70|CD63|CD81|FLOT
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20535238    
Organism Rattus norvegicus
Experiment description Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M."
Journal name Proteomics Clin Appl
Publication year 2010
Sample Urine
Sample name Urine - Rats of liver injury
Isolation/purification methods Differential centrifugation
Filtration
UltracentrifugationSucrose cushion
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Nme1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here