Gene description for Nme1
Gene name NME/NM23 nucleoside diphosphate kinase 1
Gene symbol Nme1
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Nme1
ExoCarta ExoCarta_191575
Entrez Gene 191575
UniProt Q05982  
 Nme1 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Urine 20535238    
 Gene ontology annotations for Nme1
Molecular Function
    magnesium ion binding GO:0000287 ISO
    ATP binding GO:0005524 ISO
    identical protein binding GO:0042802 ISO
    intermediate filament binding GO:0019215 IPI
    protein kinase binding GO:0019901 IPI
    RNA polymerase II regulatory region sequence-specific DNA binding GO:0000977 IDA
    nucleoside diphosphate kinase activity GO:0004550 ISO
    enzyme binding GO:0019899 IPI
    GTP binding GO:0005525 ISO
    ribosomal small subunit binding GO:0043024 ISO
    poly(A) RNA binding GO:0044822 ISO
    deoxyribonuclease activity GO:0004536 ISO
    single-stranded DNA binding GO:0003697 IDA
    protein binding GO:0005515 IPI
    gamma-tubulin binding GO:0043015 IDA
Biological Process
    cellular response to fatty acid GO:0071398 IEP
    positive regulation of neuron projection development GO:0010976 IMP
    nucleoside triphosphate biosynthetic process GO:0009142 IBA
    negative regulation of myeloid leukocyte differentiation GO:0002762 IDA
    response to drug GO:0042493 IEP
    DNA metabolic process GO:0006259 ISO
    DNA catabolic process GO:0006308 IEA
    nucleoside diphosphate phosphorylation GO:0006165 IDA
    pyrimidine nucleotide metabolic process GO:0006220 IBA
    UTP biosynthetic process GO:0006228 IEA
    response to testosterone GO:0033574 IEP
    response to amine GO:0014075 IEP
    cellular response to glucose stimulus GO:0071333 IEP
    mammary gland development GO:0030879 ISO
    regulation of apoptotic process GO:0042981 IBA
    lactation GO:0007595 ISO
    endocytosis GO:0006897 IEA
    GTP biosynthetic process GO:0006183 IEA
    response to cAMP GO:0051591 IEP
    hippocampus development GO:0021766 IEP
    positive regulation of epithelial cell proliferation GO:0050679 ISO
    CTP biosynthetic process GO:0006241 IEA
    cellular response to drug GO:0035690 IEP
    negative regulation of gene expression GO:0010629 IDA
    purine nucleotide metabolic process GO:0006163 IBA
    positive regulation of DNA binding GO:0043388 ISO
Subcellular Localization
    intermediate filament GO:0005882 IDA
    mitochondrion GO:0005739 ISO
    nucleus GO:0005634 ISO
    extracellular exosome GO:0070062 ISO
    intracellular GO:0005622 IBA
    perinuclear region of cytoplasm GO:0048471 IDA
    centrosome GO:0005813 IDA
    ruffle membrane GO:0032587 ISO
    cytosol GO:0005829 ISO
    membrane GO:0016020 ISO
    myelin sheath GO:0043209 ISO
    mitochondrial outer membrane GO:0005741 IDA
 Experiment description of studies that identified Nme1 in exosomes
1
Experiment ID 96
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 192
ISEV standards
CEM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
CD63|CD81|FLOT
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20535238    
Organism Rattus norvegicus
Experiment description Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
Authors Conde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M.
Journal name Proteomics Clin Appl
Publication year 2010
Sample Urine
Sample name Urine - Rats of liver injury
Isolation/purification methods Differential centrifugation
Filtration
UltracentrifugationSucrose cushion
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Nme1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Nme1 is involved
PathwayEvidenceSource
Synthesis and interconversion of nucleotide di- and triphosphates IEA Reactome





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