Gene description for Polr2m
Gene name polymerase (RNA) II (DNA directed) polypeptide M
Gene symbol Polr2m
Other names/aliases Glurr
Grinl1a
Species Rattus norvegicus
 Database cross references - Polr2m
ExoCarta ExoCarta_192147
Entrez Gene 192147
UniProt Q91XQ4  
 Polr2m identified in exosomes derived from the following tissue/cell type
Pancreatic cancer cells 20124479    
Pancreatic cancer cells 20124479    
 Gene ontology annotations for Polr2m
Molecular Function
    DNA-directed RNA polymerase activity GO:0003899 IEA
Biological Process
    RNA biosynthetic process GO:0032774 IEA
    maintenance of ER location GO:0051685 ISO
Subcellular Localization
    neuronal cell body GO:0043025 ISO
    nuclear envelope GO:0005635 ISO
    DNA-directed RNA polymerase II, holoenzyme GO:0016591 ISS
 Experiment description of studies that identified Polr2m in exosomes
1
Experiment ID 92
ISEV standards
EM
EV Biophysical techniques
HSP70|GAPDH
EV Cytosolic markers
CD9|CD151
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M.
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
RatRef-12 Microarray
FACS
Mass spectrometry [MALDI TOF]
2
Experiment ID 93
ISEV standards
EM
EV Biophysical techniques
HSP70|GAPDH
EV Cytosolic markers
CD9|CD151
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M.
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS-Tspan8
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
 Protein-protein interactions for Polr2m
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Polr2m is involved
No pathways found





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