Gene description for Polr2m
Gene name polymerase (RNA) II (DNA directed) polypeptide M
Gene symbol Polr2m
Other names/aliases Glurr
Grinl1a
Species Rattus norvegicus
 Database cross references - Polr2m
ExoCarta ExoCarta_192147
Vesiclepedia VP_192147
Entrez Gene 192147
UniProt Q91XQ4  
 Polr2m identified in sEVs derived from the following tissue/cell type
Pancreatic cancer cells 20124479    
Pancreatic cancer cells 20124479    
 Gene ontology annotations for Polr2m
Molecular Function
    RNA polymerase II complex binding GO:0000993 ISO
    transcription elongation factor activity GO:0003711 IEA
Biological Process
    transcription elongation by RNA polymerase II GO:0006368 IEA
    maintenance of ER location GO:0051685 IBA
    maintenance of ER location GO:0051685 ISO
Subcellular Localization
    nucleus GO:0005634 IEA
    nuclear envelope GO:0005635 ISO
    RNA polymerase II, core complex GO:0005665 IBA
    RNA polymerase II, core complex GO:0005665 ISO
    I band GO:0031674 IBA
    neuronal cell body GO:0043025 ISO
    transcription preinitiation complex GO:0097550 ISO
    transcription preinitiation complex GO:0097550 ISS
 Experiment description of studies that identified Polr2m in sEVs
1
Experiment ID 92
MISEV standards
EM
Biophysical techniques
HSP70|GAPDH|CD9|CD151
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors "Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M."
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
RatRef-12 Microarray
FACS
Mass spectrometry [MALDI TOF]
2
Experiment ID 93
MISEV standards
EM
Biophysical techniques
HSP70|GAPDH|CD9|CD151
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors "Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M."
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS-Tspan8
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
 Protein-protein interactions for Polr2m
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Polr2m is involved
No pathways found





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