Gene description for Unc13d
Gene name unc-13 homolog D (C. elegans)
Gene symbol Unc13d
Other names/aliases Munc13-4
Unc13h4
Species Rattus norvegicus
 Database cross references - Unc13d
ExoCarta ExoCarta_192177
Vesiclepedia VP_192177
Entrez Gene 192177
UniProt Q9R189  
 Unc13d identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Unc13d
Molecular Function
    small GTPase binding GO:0031267 ISO
    metal ion binding GO:0046872 IEA
Biological Process
    granuloma formation GO:0002432 IEA
    granuloma formation GO:0002432 ISO
    germinal center formation GO:0002467 IEA
    germinal center formation GO:0002467 ISO
    phagocytosis GO:0006909 IEA
    phagocytosis GO:0006909 ISO
    regulation of mast cell degranulation GO:0043304 ISO
    natural killer cell degranulation GO:0043320 IEA
    natural killer cell degranulation GO:0043320 ISO
    positive regulation of exocytosis GO:0045921 ISO
    secretion GO:0046903 IBA
    secretion GO:0046903 IEP
    defense response to virus GO:0051607 IEA
    defense response to virus GO:0051607 ISO
    establishment of localization in cell GO:0051649 ISO
    dense core granule priming GO:0061789 IGI
    positive regulation of substrate adhesion-dependent cell spreading GO:1900026 IEA
    positive regulation of substrate adhesion-dependent cell spreading GO:1900026 ISO
    positive regulation of regulated secretory pathway GO:1903307 ISO
Subcellular Localization
    lysosome GO:0005764 ISO
    late endosome GO:0005770 IEA
    cytosol GO:0005829 ISO
    membrane GO:0016020 IEA
    Weibel-Palade body GO:0033093 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
    recycling endosome GO:0055037 IEA
    exocytic vesicle GO:0070382 IBA
    exocytic vesicle GO:0070382 ISO
 Experiment description of studies that identified Unc13d in sEVs
1
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Unc13d
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Unc13d is involved
PathwayEvidenceSource
Immune System IEA Reactome
Innate Immune System IEA Reactome
Neutrophil degranulation IEA Reactome





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