ExoCarta: Gene summary

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Gene description for Nip7
Gene name	NIP7, nucleolar pre-rRNA processing protein
Gene symbol	Nip7
Other names/aliases	CGI-37 Nip7p pEachy
Species	Rattus norvegicus
Database cross references - Nip7
ExoCarta	ExoCarta_192180
Vesiclepedia	VP_192180
Entrez Gene	192180
UniProt	Q9WV50
Nip7 identified in sEVs derived from the following tissue/cell type
Pancreatic cancer cells	20124479
Pancreatic cancer cells	20124479

Gene ontology annotations for Nip7

Molecular Function
	RNA binding	GO:0003723	IEA
Biological Process
	ribosome assembly	GO:0042255	IEA
	ribosomal large subunit biogenesis	GO:0042273	IBA
Subcellular Localization
	nucleoplasm	GO:0005654	ISO
	nucleolus	GO:0005730	IBA
	nucleolus	GO:0005730	ISO
	cytosol	GO:0005829	ISO
	preribosome, large subunit precursor	GO:0030687	IBA

Experiment description of studies that identified Nip7 in sEVs

Experiment ID

MISEV standards
✔ EM	Biophysical techniques
✔ HSP70\|GAPDH\|CD9\|CD151	Enriched markers
✘	Negative markers
✘	Particle analysis

Identified molecule

mrna

Identification method

Microarray

PubMed ID

20124479

Organism

Rattus norvegicus

Experiment description

Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.

Authors

"Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M."

Journal name

Publication year

2010

Sample

Pancreatic cancer cells

Sample name

Isolation/purification methods

Differential centrifugation
Sucrose density gradient

Flotation density

1.14-1.17 g/mL

Molecules identified in the study

Protein
mRNA

Methods used in the study

RT-PCR
Western blotting
RatRef-12 Microarray
FACS
Mass spectrometry [MALDI TOF]