Gene description for Slc38a5
Gene name solute carrier family 38, member 5
Gene symbol Slc38a5
Other names/aliases SN2
Species Rattus norvegicus
 Database cross references - Slc38a5
ExoCarta ExoCarta_192208
Vesiclepedia VP_192208
Entrez Gene 192208
UniProt A2VCW5  
 Slc38a5 identified in sEVs derived from the following tissue/cell type
Pancreatic cancer cells 20124479    
Pancreatic cancer cells 20124479    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Slc38a5
Molecular Function
    L-histidine transmembrane transporter activity GO:0005290 IDA
    L-histidine transmembrane transporter activity GO:0005290 ISO
    amino acid transmembrane transporter activity GO:0015171 IDA
    neutral L-amino acid transmembrane transporter activity GO:0015175 IDA
    L-asparagine transmembrane transporter activity GO:0015182 ISO
    L-glutamine transmembrane transporter activity GO:0015186 IBA
    L-glutamine transmembrane transporter activity GO:0015186 IDA
    L-glutamine transmembrane transporter activity GO:0015186 ISO
    glycine transmembrane transporter activity GO:0015187 IBA
    glycine transmembrane transporter activity GO:0015187 IDA
    glycine transmembrane transporter activity GO:0015187 ISO
    L-serine transmembrane transporter activity GO:0015194 IDA
    L-serine transmembrane transporter activity GO:0015194 ISO
    alanine transmembrane transporter activity GO:0022858 ISO
    L-glutamine, sodium:proton antiporter activity GO:0140830 IDA
    L-glutamine, sodium:proton antiporter activity GO:0140830 IEA
    L-glutamine, sodium:proton antiporter activity GO:0140830 ISO
    L-glutamine, sodium:proton antiporter activity GO:0140830 ISS
    neutral amino acid, sodium:proton antiporter activity GO:0140893 IDA
    neutral amino acid, sodium:proton antiporter activity GO:0140893 ISO
    neutral amino acid, sodium:proton antiporter activity GO:0140893 ISS
Biological Process
    asparagine transport GO:0006867 IEA
    glutamine transport GO:0006868 IBA
    glutamine transport GO:0006868 IDA
    glutamine transport GO:0006868 ISO
    neutral amino acid transport GO:0015804 IDA
    neutral amino acid transport GO:0015804 ISO
    glycine transport GO:0015816 IBA
    glycine transport GO:0015816 IDA
    glycine transport GO:0015816 ISO
    L-serine transport GO:0015825 IDA
    serine transport GO:0032329 IBA
    serine transport GO:0032329 ISO
    amino acid export across plasma membrane GO:0032973 IDA
    sodium ion transmembrane transport GO:0035725 IEA
    L-histidine transmembrane transport GO:0089709 IBA
    L-histidine transmembrane transport GO:0089709 IDA
    L-histidine transmembrane transport GO:0089709 ISO
    amino acid import across plasma membrane GO:0089718 IDA
    monoatomic cation transmembrane transport GO:0098655 IEA
    inorganic cation transmembrane transport GO:0098662 IEA
    asparagine transmembrane transport GO:1903713 ISO
    L-glutamine import across plasma membrane GO:1903803 IDA
    L-glutamine import across plasma membrane GO:1903803 IEA
    L-glutamine import across plasma membrane GO:1903803 ISO
    L-glutamine import across plasma membrane GO:1903803 ISS
    L-serine import across plasma membrane GO:1903812 IDA
    L-alanine transmembrane transport GO:1904557 ISO
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    membrane GO:0016020 IEA
 Experiment description of studies that identified Slc38a5 in sEVs
1
Experiment ID 92
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|GAPDH|CD9|CD151
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors "Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M."
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
RatRef-12 Microarray
FACS
Mass spectrometry [MALDI TOF]
2
Experiment ID 93
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|GAPDH|CD9|CD151
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors "Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M."
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS-Tspan8
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
3
Experiment ID 90
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70|HSP90|TSG101|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 94
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Slc38a5
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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