Gene description for Eif2b5
Gene name eukaryotic translation initiation factor 2B, subunit 5 epsilon
Gene symbol Eif2b5
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Eif2b5
ExoCarta ExoCarta_192234
Entrez Gene 192234
UniProt Q64350  
 Eif2b5 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Eif2b5
Molecular Function
    protein binding GO:0005515 IPI
    translation initiation factor activity GO:0003743 TAS
    guanyl-nucleotide exchange factor activity GO:0005085 ISS
    translation initiation factor binding GO:0031369 IMP
Biological Process
    ovarian follicle development GO:0001541 ISS
    response to heat GO:0009408 ISS
    myelination GO:0042552 ISS
    translational initiation GO:0006413 TAS
    cellular response to drug GO:0035690 ISS
    response to peptide hormone GO:0043434 IDA
    astrocyte differentiation GO:0048708 ISS
    positive regulation of translational initiation GO:0045948 ISS
    negative regulation of translational initiation in response to stress GO:0032057 IMP
    response to glucose GO:0009749 IDA
    astrocyte development GO:0014002 ISS
    oligodendrocyte development GO:0014003 ISS
    response to endoplasmic reticulum stress GO:0034976 ISS
    positive regulation of GTPase activity GO:0043547 ISS
    regulation of translation GO:0006417 IMP
Subcellular Localization
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 ISS
    eukaryotic translation initiation factor 2B complex GO:0005851 TAS
 Experiment description of studies that identified Eif2b5 in exosomes
1
Experiment ID 95
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Eif2b5
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Eif2b5 is involved
PathwayEvidenceSource
Recycling of eIF2:GDP IEA Reactome





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