Gene description for Sub1
Gene name SUB1 homolog (S. cerevisiae)
Gene symbol Sub1
Other names/aliases Rpo2tc1
Species Rattus norvegicus
 Database cross references - Sub1
ExoCarta ExoCarta_192269
Entrez Gene 192269
UniProt Q63396  
 Sub1 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Sub1
Molecular Function
    poly(A) RNA binding GO:0044822 ISO
    molecular_function GO:0003674 ND
    RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription GO:0001205 ISO
    transcription coactivator activity GO:0003713 ISO
    single-stranded DNA binding GO:0003697 ISO
Biological Process
    biological_process GO:0008150 ND
    SMAD protein signal transduction GO:0060395 ISO
    transcription from RNA polymerase II promoter GO:0006366 ISO
    regulation of transcription from RNA polymerase II promoter GO:0006357 ISO
    transcription, DNA-templated GO:0006351 IEA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 ISO
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleolus GO:0005730 ISO
    extracellular exosome GO:0070062 ISO
    cellular_component GO:0005575 ND
    transcription factor complex GO:0005667 ISO
 Experiment description of studies that identified Sub1 in exosomes
1
Experiment ID 96
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Sub1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Sub1 is involved
No pathways found





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