Gene description for Sub1
Gene name SUB1 homolog (S. cerevisiae)
Gene symbol Sub1
Other names/aliases Rpo2tc1
Species Rattus norvegicus
 Database cross references - Sub1
ExoCarta ExoCarta_192269
Vesiclepedia VP_192269
Entrez Gene 192269
UniProt Q63396  
 Sub1 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Sub1
Molecular Function
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IEA
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 ISO
    DNA helicase activity GO:0003678 ISO
    double-stranded DNA binding GO:0003690 ISO
    single-stranded DNA binding GO:0003697 ISO
    transcription coactivator activity GO:0003713 IBA
    transcription coactivator activity GO:0003713 IEA
    transcription coactivator activity GO:0003713 ISO
    identical protein binding GO:0042802 ISO
    DNA-binding transcription factor binding GO:0140297 ISO
Biological Process
    RNA polymerase II promoter clearance GO:0001111 ISO
    regulation of transcription by RNA polymerase II GO:0006357 ISO
    biological_process GO:0008150 ND
    DNA duplex unwinding GO:0032508 ISO
    negative regulation of DNA metabolic process GO:0051053 ISO
    protein homooligomerization GO:0051260 ISO
    positive regulation of transcription initiation by RNA polymerase II GO:0060261 IEA
    negative regulation of DNA duplex unwinding GO:1905463 ISO
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IEA
    nucleoplasm GO:0005654 ISO
    transcription regulator complex GO:0005667 IBA
    transcription regulator complex GO:0005667 ISO
    nucleolus GO:0005730 ISO
 Experiment description of studies that identified Sub1 in sEVs
1
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Sub1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tp53  
Affinity Capture-Western Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
View the network image/svg+xml
 Pathways in which Sub1 is involved
No pathways found





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