Gene description for Dad1
Gene name defender against cell death 1
Gene symbol Dad1
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Dad1
ExoCarta ExoCarta_192275
Entrez Gene 192275
UniProt P61805  
 Dad1 identified in exosomes derived from the following tissue/cell type
Adipocytes 25998041    
 Gene ontology annotations for Dad1
Molecular Function
    oligosaccharyl transferase activity GO:0004576 ISS
    dolichyl-diphosphooligosaccharide-protein glycotransferase activity GO:0004579 IEA
    molecular_function GO:0003674 ND
Biological Process
    protein glycosylation GO:0006486 ISS
    blastocyst development GO:0001824 ISO
    response to nutrient GO:0007584 IEP
    apoptotic process GO:0006915 IEA
    response to drug GO:0042493 IEP
    negative regulation of apoptotic process GO:0043066 ISO
Subcellular Localization
    extracellular exosome GO:0070062 ISO
    membrane GO:0016020 ISO
    cellular_component GO:0005575 ND
    oligosaccharyltransferase complex GO:0008250 ISS
    integral component of membrane GO:0016021 IEA
 Experiment description of studies that identified Dad1 in exosomes
1
Experiment ID 225
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors Lee JE, Moon PG, Lee IK, Baek MC
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for Dad1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Dad1 is involved
No pathways found





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