Gene description for Eml2
Gene name echinoderm microtubule associated protein like 2
Gene symbol Eml2
Other names/aliases Emap
Emap-2
Eml1
Species Rattus norvegicus
 Database cross references - Eml2
ExoCarta ExoCarta_192360
Vesiclepedia VP_192360
Entrez Gene 192360
UniProt Q6P6T4  
 Eml2 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Eml2
Molecular Function
    microtubule binding GO:0008017 IBA
    microtubule binding GO:0008017 IEA
    microtubule binding GO:0008017 ISO
    microtubule binding GO:0008017 ISS
    tubulin binding GO:0015631 ISO
    tubulin binding GO:0015631 ISS
    ionotropic glutamate receptor binding GO:0035255 IDA
Biological Process
    microtubule cytoskeleton organization GO:0000226 IBA
    regulation of microtubule nucleation GO:0010968 IEA
    regulation of microtubule nucleation GO:0010968 ISO
    regulation of microtubule nucleation GO:0010968 ISS
    negative regulation of microtubule polymerization GO:0031115 IEA
    negative regulation of microtubule polymerization GO:0031115 ISO
    negative regulation of microtubule polymerization GO:0031115 ISS
Subcellular Localization
    cytoplasm GO:0005737 IEA
    microtubule GO:0005874 IEA
    membrane GO:0016020 IEA
    mitotic spindle GO:0072686 IBA
    mitotic spindle GO:0072686 IEA
    mitotic spindle GO:0072686 ISO
    mitotic spindle GO:0072686 ISS
 Experiment description of studies that identified Eml2 in sEVs
1
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Eml2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Eml2 is involved
No pathways found





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