ExoCarta: Gene summary

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Gene description for Eml2
Gene name	echinoderm microtubule associated protein like 2
Gene symbol	Eml2
Other names/aliases	Emap Emap-2 Eml1
Species	Rattus norvegicus
Database cross references - Eml2
ExoCarta	ExoCarta_192360
Entrez Gene	192360
UniProt	Q6P6T4
Eml2 identified in exosomes derived from the following tissue/cell type
Reticulocytes	21828046
Reticulocytes	21828046

Gene ontology annotations for Eml2

Molecular Function
	tubulin binding	GO:0015631	ISS
	catalytic activity	GO:0003824	IEA
	protein C-terminus binding	GO:0008022	IDA
	microtubule binding	GO:0008017	ISS
	receptor binding	GO:0005102	IDA
Biological Process
	metabolic process	GO:0008152	IEA
	negative regulation of microtubule polymerization	GO:0031115	ISS
	regulation of microtubule nucleation	GO:0010968	ISS
Subcellular Localization
	mitotic spindle	GO:0072686	ISS
	microtubule	GO:0005874	IEA
	cytoplasm	GO:0005737	IEA

Experiment description of studies that identified Eml2 in exosomes

Experiment ID

ISEV standards
✔ EM	EV Biophysical techniques
✔ TSG101\|HSP90\|HSC70	EV Cytosolic markers
✔ MHCI	EV Membrane markers
✘	EV Negative markers
✘	EV Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry [Orbitrap]

PubMed ID

21828046

Organism

Rattus norvegicus

Experiment description

The proteo-lipidic composition of exosomes changes during reticulocyte maturation.

Authors

Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.

Journal name

JBC

Publication year

2011

Sample

Reticulocytes

Sample name

Reticulocytes - D7 Rep 1

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.13-1.25 g/mL

Molecules identified in the study

Protein
Lipids

Methods used in the study

Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting