Gene description for Purb
Gene name purine rich element binding protein B
Gene symbol Purb
Other names/aliases 2310015K15Rik
AA114818
Cager-2
D11Bwg0414e
Species Mus musculus
 Database cross references - Purb
ExoCarta ExoCarta_19291
Vesiclepedia VP_19291
Entrez Gene 19291
UniProt O35295  
 Purb identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Purb
Molecular Function
    DNA binding GO:0003677 IEA
    SMAD binding GO:0046332 IDA
    translation repressor activity, nucleic acid binding GO:0000900 ISO
    purine-rich negative regulatory element binding GO:0032422 ISO
    sequence-specific DNA binding transcription factor activity GO:0003700 IDA
    RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription GO:0001227 IDA
    double-stranded DNA binding GO:0003690 IDA
    mRNA binding GO:0003729 IDA
    poly(A) RNA binding GO:0044822 ISO
    RNA binding GO:0003723 IDA
    single-stranded DNA binding GO:0003697 ISO
    transcription factor binding GO:0008134 IPI
    RNA polymerase II regulatory region sequence-specific DNA binding GO:0000977 IDA
    protein binding GO:0005515 IPI
Biological Process
    cell differentiation GO:0030154 NAS
    negative regulation of translation GO:0017148 ISO
    apoptotic process GO:0006915 NAS
    negative regulation of transcription, DNA-templated GO:0045892 ISO
    regulation of transcription, DNA-templated GO:0006355 IEA
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IGI
    cell proliferation GO:0008283 NAS
    transcription, DNA-templated GO:0006351 IEA
    transcription from RNA polymerase II promoter GO:0006366 IDA
Subcellular Localization
    nucleoplasm GO:0005654 ISO
    nucleus GO:0005634 ISO
    DNA replication factor A complex GO:0005662 IDA
 Experiment description of studies that identified Purb in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Purb
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Purb is involved
No pathways found





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